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EC number: 204-977-6 | CAS number: 130-15-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on test solutions:
- Test substance stock solutions: A 200 mg sample of the test substance was ground in a mortar and dissolved in ultra pure water (2 L). This was stirred for 5 minutes, sonicated for 5 minutes to prepare a 100 mg/L stock solution.
Reference substance stock solution: A 200 mg sample of DCP dissolved in ultra pure water (200 mL, 1000 mg/L). This was then used to prepare a 100 mg/L stock solution.
Nitrification inhibitor stock solutions: A 464 mg sample of ATU was dissolved in ultra pure water (200 mL, 2320 mg/L).
The pH of all above stock solutions were checked and adjusted by adding NaOH and H2SO4 to pH 7.5 ± 0.5. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge sourced from a domestic sewage treatment plant. Sludge collected 2 days before exposure and stored aerated at 20 ± 2°C. The MLSS was determined to be 2970 mg/L with a pH of 7.5 ± 0..5. The sewage was collected without sieving and washing. The synthetic sewage feed was added on the day of collection The MLSS was adjusted to 3000 mg/L on the day of exposure.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- Not determined
- Test temperature:
- 20.2 to 20.3°C (in water tank)
- pH:
- Controls: Start 7.2, end 7.9 to 8.
Test mixtures: Start 7.2 to 7.3, end 7.8 to 8.
Reference mixtures: 7.2 to 7.3, end 7.8 to 8. - Dissolved oxygen:
- Varies per vessel and is main endpoint assessed
- Salinity:
- Not determined
- Conductivity:
- Not determined
- Nominal and measured concentrations:
- Test solutions: 0.1, 0.42, 1.7, 7.2 and 30 mg/L
Reference mixtures: 0.1, 0.5, 3.2, 18 and 100 mg/L - Details on test conditions:
- TEST SYSTEM
See 'other information on materials and methods incl. tables' for full details.
- Test vessel: 1000 L (glass)
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 500 mL
- Aeration: Yes
- Sludge concentration (weight of dry solids per volume): 30 mg/L
- Nitrification inhibitor used: N-allylthiourea
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Ultra pure water
OTHER TEST CONDITIONS
- Adjustment of pH: Test stock solutions adjusted
- Photoperiod: Light for 3 hours
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Respiration inhibition based on DO.
TEST CONCENTRATIONS
- Range finding study: Conducted at 0.01, 0.1, 1, 10, 100 and 1000 mg/L. This showed high respiration inhibition at > 1 mg/L.
- Test concentrations:0.1, 0.42, 1.7, 7.2 and 30 mg/L
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (DCP)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.94 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.84 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.72 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.28 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 3.42 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of respiration due to nitrification
- Details on results:
- The average inhibition rates at the relevant concentrations are as follows:
Average inhibition (total respiration): -7.2, 9.2, 9.2, 59.6 and 94.7% (at 0.1, 0.42, 1.7, 7.2 and 30 mg/L, respectively).
Average inhibition (heterotrophic respiration): 6.7, 9.6, 14.7, 67 and 95.3% (at 0.1, 0.42, 1.7, 7.2 and 30 mg/L, respectively).
Average inhibition (nitrification respiration): -46.2, 8.1, -6.6, 39.3 and 93.4% (at 0.1, 0.42, 1.7, 7.2 and 30 mg/L, respectively).
The specific respiration rate of the controls was 28.1 mg oxygen per gram of activated sludge (dry weight of suspended solids) in an hour.
The CoV of oxygen consumption rate in control replicates was 9.7% - Results with reference substance (positive control):
- The EC50 of DCP was 10.9, 12.6 and 2.26 mg/L for total, heterotrophic and nitrification respiration, respectively. This is within the guideline ranges indicating the test is valid.
- Reported statistics and error estimates:
- ECx: If the percentage inhibition is greater than 50%, the EC50 value is calculated using a probit method, which was conducted in this case (Ecotox Statics ver.2.6d)
NOEC: This was not determined as the EC50 was determined. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of an ASRIT study conducted in accordance with the OECD 209 guideline, 3 hour EC50 values of 5.94, 4.84 and 9.16 mg/L were determined for total respiration, heterotrophic respiration and nitrification respiration, respectively. No NOEC was determined but EC10 values are available.
Reference
Description of key information
Under the conditions of an ASRIT study conducted in accordance with the OECD 209 guideline, 3 hour EC50 values of 5.94, 4.84 and 9.16 mg/L were determined for total respiration, heterotrophic respiration and nitrification respiration, respectively. The value for total respiration is selected as the key value for chemical safety assessment. No NOEC was determined but EC10 values are available. The EC10 value for total respiration has been included as the key value for chemical safety assessment.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 5.94 mg/L
- EC10 or NOEC for microorganisms:
- 1.72 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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