[[4-[[2-(4-cyclohexylphenoxy)ethyl]ethylamino]-2-methylphenyl]methylene]malononitrile

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental report according to guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Qualifier:

according to guideline

Guideline:

other: Official Journal of the EC L 133 Part C

Principles of method if other than guideline:

Examination of the respiratory inhibition of activated sludge for test material

GLP compliance:

not specified

Details on sampling:

Direct weighing concentration of the animated mud in d. Prüfans. : 320.0 mg / l

Test organisms (species):

activated sludge

Details on inoculum:

activated sludge (mixed population different microorganisms)
Origin: Laboratory plant mainly with municipal sewage
Pretreatment: none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Remarks on exposure duration:

30 minutes under constant ventilation

Test temperature:

20 ± 2 ° C

pH:

7.7

Nominal and measured concentrations:

1000 , 1800 , 3200 , 5600 , 10000 mg/l

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorphenol

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

Based on the results obtained for test material the following toxicity to activated sludge EC 50> 10000 mg / l

Results with reference substance (positive control):

3,5-Dichlorphenol EC50 = 8.8 mg/l

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 valueof test material was observed to be >10000 mg/l after 30 min of respiration inhibition test.

Executive summary:

Effect of test material on activated sludge was evaluated according to Official Journal of the EC L 133 Part C: Biodegradability: examination of the respiratory Inhibition (largely corresponds to the test method OECD 209).A defined amount of activated sludge is made with synthetic Medium offset and measured the respiratory rate. These Respiration rate is compared with those used in test with different concentrations of the test substance be measured. The sensitivity of the activated sludge used is checked with 3,5-dichlorophenol as reference substance. Activated sludge (mixed population different microorganisms)were obtained from Laboratory plant mainly with municipal sewage. The EC50 valueof test material was observed to be >10000 mg/l after 30 min of respiration inhibition test. 

Description of key information

Toxicity to microorganism:

Effect of test material on activated sludge was evaluated according to Official Journal of the EC L 133 Part C: Biodegradability: examination of the respiratory Inhibition (largely corresponds to the test method OECD 209).A defined amount of activated sludge is made with synthetic Medium offset and measured the respiratory rate. These Respiration rate is compared with those used in test with different concentrations of the test substance be measured. The sensitivity of the activated sludge used is checked with 3,5-dichlorophenol as reference substance. Activated sludge (mixed population different microorganisms)were obtained from Laboratory plant mainly with municipal sewage. The EC50 valueof test material was observed to be >10000 mg/l after 30 min of respiration inhibition test. 

The test material is highly insoluble in water , the solubility was observed to be < 0.0007 mg/L , hence indicating that aquatic toxicity is unliky to occure.

Key value for chemical safety assessment

EC50 for microorganisms:

10 000 mg/L

Additional information

Toxicity to microorganism:

Effect of test material on activated sludge was evaluated according to Official Journal of the EC L 133 Part C: Biodegradability: examination of the respiratory Inhibition (largely corresponds to the test method OECD 209).A defined amount of activated sludge is made with synthetic Medium offset and measured the respiratory rate. These Respiration rate is compared with those used in test with different concentrations of the test substance be measured. The sensitivity of the activated sludge used is checked with 3,5-dichlorophenol as reference substance. Activated sludge (mixed population different microorganisms)were obtained from Laboratory plant mainly with municipal sewage. The EC50 valueof test material was observed to be >10000 mg/l after 30 min of respiration inhibition test. 

The test material is highly insoluble in water , the solubility was observed to be < 0.0007 mg/L , hence indicating that aquatic toxicity is unliky to occure.