2,4-dichloroaniline

Toxicity to soil microorganisms

Administrative data

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Persistence and effects of some chlorinated anilines on nitrification in soil.

Author:

Thompson FR and Corke CT

Year:

1969

Bibliographic source:

Can. J. Microbiol. 15, 791-796.

Reference 2

Reference Type:

secondary source

Title:

2,4-Dichloranilin; 2,5-Dichloranilin; 3,4-Dichloranilin

Author:

Beratergremium für umweltrelevante Altstoffe (BUA)

Year:

1994

Bibliographic source:

Wissenschaftliche Verlagsgesellschaft, BUA report 140

Materials and methods

Principles of method if other than guideline:

The toxicity against nitrifying bacteria was tested by measuring the effect of 2,4-dichloroaniline on the oxidation of ammonium-nitrogen in a Guelph loam. The soil perfusion technique was applied (Chase F. E., 1948).

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dichloroaniline

EC Number:

209-057-8

EC Name:

2,4-dichloroaniline

Cas Number:

554-00-7

Molecular formula:

C6H5Cl2N

IUPAC Name:

2,4-dichloroaniline

Sampling and analysis

Analytical monitoring:

no

Test substrate

Details on preparation and application of test substrate:

- soil - type: Guelph loam
- soil - pH: 7.0-7.4
- soil - test volume: 50 g air dried loam

Test conditions

Nominal and measured concentrations:

nominal concentration: 100 µg 2,4-dichloroaniline / g soil

Results and discussion

Any other information on results incl. tables

2,4-dichloroaniline (100 µg/ g soil)

time for complete oxidation of NH4-N to NO3-N => 62 days

 

control:

time for complete oxidation of NH4-N to NO3-N => 10 days

 

The experiments with the addition of NO₂^-showed that the second step of nitrification is not inhibited by dichloroaniline. Thus, the inhibition of nitrification is based on the inhibition of the oxidation of NH₄⁺to NO₂^-. 2,4-dichloronaniline produced a lag period of 46 days prior to the start of the nitrification process. Once the nitrification was started the reaction rate was not affected by the test substance.