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EC number: 700-490-2 | CAS number: 36635-56-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- N-[[(4-methylphenyl)sulfonyl]methyl]formamide
- EC Number:
- 700-490-2
- Cas Number:
- 36635-56-0
- Molecular formula:
- C9H11NO3S
- IUPAC Name:
- N-[[(4-methylphenyl)sulfonyl]methyl]formamide
- Details on test material:
- - Name of test material (as cited in study report): CGA 224433
- Physical state: solid
- Analytical purity: ca 100%
- Lot/batch No.: P. 709027
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- Toxicity test:
9.77 - 5000.0µg/ml range
Without activation:
125.0 - 1000.0µg/ml range
With microsomal activation:
200.0 - 1600.0µg/ml range - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- Remarks:
- harvesting time 21hrs only
Migrated to IUCLID6: 40.0µg/ml
- Untreated negative controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- mitomycin C
- Remarks:
- harvesting time 21hrs only
Migrated to IUCLID6: 1.0µg/ml
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar (plate incorporation); preincubation; in suspension; as impregnation on paper disk
DURATION
- Preincubation period: 22hrs
- Exposure duration: 3hrs
- Expression time (cells in growth medium): 4hrs and 21hrs
DETERMINATION OF CYTOTOXICITY
- Method: mitotic suppression - Evaluation criteria:
- Criteria for positive response:
- a test substance is considered to be active if, in comparison to the negative control, a marked increase in the number of specific chromosomal aberrations appear or if an increased number of exchange figures appear together with a high number of other specific chromosomal aberrations such as breaks or fragments
- a concentration-related response in the number of aberrations should be demonstrable
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- with and without activation
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive with metabolic activation 21hrs only
positive without metabolic activation both 4hrs and 21hrs
In the experiment with a harvesting time of 4 hours performed without microsomal activation, at concentrations of 1000µg/ml an increase in specific chromosomal aberrations was registered.
In the experiment with a harvesting time of 21 hours performed without microsomal activation, at concentrations of 1000µg/ml a marked increase in specific chromosomal aberrations was detected (23%).
In the experiment with a harvesting time of 21 hours performed with microsomal activation, at concentrations of 1600µg/ml 19% of metaphases with specific chromosomal aberrations was detected, thereof 9% containing exchange figures.
Thus the results indicate that under the given experimental conditions CGA 224433, in 21hrs with and without activation and 4hrs without activation, exerted a mutagenic effect in Chinese hamster ovary cells in vitro. - Executive summary:
CGA 224433 under test conditions was seen to exert a mutagenic effect on the tested cells and thus suggests this substance is a cause of concern for man owing to possible carcinogenic effects and should be assign the appropriate hazard phrases
EU classification R40 limited evidence of carcenogenic effect
GHS classifiaction Category 2 suspected human carcinogen.
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