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EC number: 212-485-8 | CAS number: 822-06-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- before July 1970
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 970
- Report date:
- 1970
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- no data on body weights or necropsy findings
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Hexamethylene diisocyanate
- EC Number:
- 212-485-8
- EC Name:
- Hexamethylene diisocyanate
- Cas Number:
- 822-06-0
- Molecular formula:
- C8H12N2O2
- IUPAC Name:
- 1,6-diisocyanatohexane
- Reference substance name:
- Desmodur H
- IUPAC Name:
- Desmodur H
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: breeder Winkelmann (Kirchborchen, Germany)
- Age at study initiation: no data
- Weight at study initiation: 160-200 g
- Housing: no data
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21+/- 2
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12 /12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: oil, not specified
- Details on oral exposure:
- application volume: 5 ml/kg bw
- Doses:
- 0.1, 0.25, 0.5, 0.75, 1.0, 1.5, and 2.0 mL/kg bw corresponding to 105, 263, 525, 788, 1050, 1575, and 2100 mg/kg bw (density 1.05 g/cm³ at 25 °C)
- No. of animals per sex per dose:
- 15
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs - Statistics:
- LD50 values were calculated according to the method of Litchfield and Wilcoxon (J. Pharmacol. exper. Therap. 96: 99, 1949).
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 959 mg/kg bw
- 95% CL:
- >= 837 - <= 1 103
- Remarks on result:
- other: corresponding to 0.913 mL/kg
- Mortality:
- Minumum lethal dose: 788 mg/kg bw
Mortalities at dose levels: 0/15 at 105 mg/kg, 0/15 at 263 mg/g, 0/15 at 525 mg/kg, 5/15 at 788 mg/kg, 11/15 at 1050 mg/kg, 14/15 at 1575 mg/kg, 15/15 at 2100 mg/kg. All deaths occurred within the first day after treatment. - Clinical signs:
- other:
- Body weight:
- other body weight observations
- Remarks:
- no data
- Gross pathology:
- no data
Any other information on results incl. tables
Table 1: Toxicological results after single oral application of HDI to male rats
Sex | Dose (mg/kg bw) | Toxicological results | Onset and duration of signs | Onset of mortality |
male | 105 | 0 / 0 / 15 | --- | --- |
263 | 0 / 15 / 15 | 2h - 3d | --- | |
525 | 0 / 15 / 15 | 1h - 7d | --- | |
788 | 5 / 15 / 15 | 50m - 7d | 1d | |
1050 | 11 / 15 / 15 | 40m - 7d | 1d | |
1575 | 14 / 15 / 15 | 35m | 1d | |
2100 | 15 / 15 / 15 | 30m | 1d |
Toxicological results:
number of dead animals / number of animals with signs after treatment / number of animals treated
Applicant's summary and conclusion
- Executive summary:
An acute oral toxicity study in male Wistar rats is available for the substance. In that study, which was conducted prior to GLP and OECD guidelines, 15 rats per dose group were administered by gavage (administration volume 5 mL/kg) with substance formulations in oil at 105, 263, 525, 788, 1050, 1575, and 2100 mg/kg bw. During a 14 -day post-dosing period animals were at least daily observed for clinical signs and mortalities. No body weights were reported and no necropsy was conducted. LD50 values were calculated according to the method of Litchfield and Wilcoxon.
The oral LD50 in this study was 959 mg/kg for male Wistar rats. At 263 mg/kg and above all animals showed a reduced general condition and a mild sedation. The NOEL with regard to mortality and clinical signs was 105 mg/kg.
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