Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 247-955-1 | CAS number: 26748-41-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001-06-11 to 2001-06-26
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1989
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: At the start of the test samples of about 15 mL were taken from the test vessels with the diluted WAF and the control, just before the distribution of the solutions over the four batches and adding the test animals. At the end of the test the solutions from the four batches were combined and mixed and 15 mL samples from the undiluted WAF and the control were used for analysis.
- Sample storage conditions before analysis: Samples were stored in a refrigerator until analyses. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
The test solution was a Water Accommodated Fraction (WAF) prepared from 1.01 g test substance in 2 liters of DSW. THis solution was slowly stirred for approximately 48 hours, then transferred into a seperation funnel and left at test temperature for phase separation. After approximately 3 hours, when phase separation was complete, the aqueous phase was withdrawn and used to prepare the following dilutions: 1:16 - 1:8 - 1:4 -1:2 and the undiluted WAF - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water fleas
- Source: Akzo Nobel Chemicals Research, Arnhem, Dept. CGS-ENV
- Age at study initiation: less than 24 hours
- Feeding during test: no feeding - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- approximately 12°dH
- Test temperature:
- 19.3 to 21 °C
- pH:
- 7.5 to 8.1 (at the start)
8.0 to 8.1 (at the end) - Dissolved oxygen:
- 8.5 to 8.6 (at the start)
8.9 to 9.0 (at the end) - Nominal and measured concentrations:
- - nominal concentrations: 0.1906, 0.3813, 0.7625, 1.525, 3.05 mg/L
- measured concentrations: The results show that the undiluted WAF contains 3.05 mg/L test item at the beginning of the test and below the detection limit (1.64 mg/L) at the end of the test, which is less than 80 % of the initial concentration. These results show that the test substance is probably not stable under test conditions. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 400 mL glass beakers containing approximately 250 mL of the test solution
- Type: closed
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dutch Standard water
- Intervals of water quality measurement: The dissolved oxygen and pH of all test concentrations and controls were measured at the beginning and at the end of the test. The temperature was measured continuously in an additional test vessel filled with deionized water and kept under identical conditions as the actual test vessels.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours per day provided by fluorescent tubes
EFFECT PARAMETERS MEASURED : The daphnias were exposed to the chosen concentrations of the test substance as described below and immobility and sub-lethal effects were recorded at approximately 24 and 48 hours. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.79 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.68 and 0.93 mg/L
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.381 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.763 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 1.525 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Sublethal effects were not observed in the concentrations where no immobility was observed.
- Reported statistics and error estimates:
- The EC50 was calculated with the computer program TOXCALC version 5.0 according to Standard Operation Procedure L2, using the trimmed Spearman-Kärber method. The concentration as measured in the WAF at the beginning of the test was used to calculate the concentrations of the dilutions. The concentrations causing zero and 100 % immobility were derived directly from the test observations.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (48 h) of tert-butyl peroxyneodecanoate was calculated to be 0.79 mg/L with 95% confidence limits of 0.68 and 0.93 mg/L.
The highest concentration causing no immobility (no observed effect concentration, NOEC) after 48 hours amounted to 0.38 mg/L, whereas 0.76 mg(L was the lowest tested concentration (LOEC, 48 h) at which significant immobility was observed as compared to the control. The EC100 was 1.53 mg/L, which was observed after 48 hours. - Executive summary:
The acute toxicity of the Water Accommodated Fraction (WAF) of tert-butyl peroxyneodecanoate to Daphnia magna (water flea) was tested under static conditions for 48 hours in accordance with OECD guideline no. 202 and EU method C.2. The test item is immiscible with water and therefore a WAF was prepared. The following dilutions of the WAF were tested: 1:16 - 1:8 - 1:4 - 1:2 and the undiluted WAF. Chemical analyses of the control and WAF were performed and showed that the test substance is probably unstable under test conditions. At beginning of the test a concentration of 3.05 mg/L was measured. At the end of the test a concentration below 1.64 mg/L was measured. The concentration at the beginning of the test was used for calculation of the effect concentrations.
The EC50 (48 h) was calculated to be 0.79 mg/L with 95% confidence limits of 0.68 and 0.93 mg/L.
The highest concentration causing no immobility (no observed effect concentration, NOEC) after 48 hours amounted to 0.38 mg/L, whereas 0.76 mg/L was the lowest tested concentration (LOEC, 48 h) at which significant immobility was observed as compared to the control. The EC100 was 1.53 mg/L, which was observed after 48 hours.
Sublethal effects were not observed in the test concentrations where no immobility was observed.
Reference
Description of key information
The acute toxicity to invertebrates was assessed according to OECD guideline 202 and EU-method C.2. In this 48-hour acute toxicity test with daphnia, the EC50 value was 0.79 mg/L (nominal concentration). The no-observed-effect concentration (NOEC) was 0.381 mg/L (nominal concentration).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.79 mg/L
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 0.381 mg/L
Additional information
The acute toxicity of the Water Accommodated Fraction (WAF) of tert-butyl peroxyneodecanoate to Daphnia magna (water flea) was tested under static conditions for 48 hours in accordance with OECD guideline no. 202 and EU method C.2. The test item is immiscible with water and therefore a WAF was prepared. The following dilutions of the WAF were tested: 1:16 - 1:8 - 1:4 - 1:2 and the undiluted WAF. Chemical analyses of the control and WAF were performed and showed that the test substance is probably unstable under test conditions. At beginning of the test a concentration of 3.05 mg/L was measured. At the end of the test a concentration below 1.64 mg/L was measured. The concentration at the beginning of the test was used for calculation of the effect concentrations. The EC50 (48 h) was calculated to be 0.79 mg/L with 95% confidence limits of 0.68 and 0.93 mg/L. The highest concentration causing no immobility (no observed effect concentration, NOEC) after 48 hours amounted to 0.38 mg/L, whereas 0.76 mg/L was the lowest tested concentration (LOEC, 48 h) at which significant immobility was observed as compared to the control. The EC100 was 1.53 mg/L, which was observed after 48 hours. Sublethal effects were not observed in the test concentrations where no immobility was observed.
.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)