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EC number: 413-550-5 | CAS number: 142068-96-0 H112339
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 December 1991 to 12 December 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to recognised EU test guideline in compliance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 84/449/EEC
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate
- EC Number:
- 413-550-5
- EC Name:
- Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate
- Cas Number:
- 142068-96-0
- Molecular formula:
- Hill formula: C53H28Cl2N14Na8O26S8
- IUPAC Name:
- octasodium 5-[(4-chloro-6-{[4-({[4-chloro-6-({7-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-8-hydroxy-3,6-disulfonatonaphthalen-1-yl}amino)-1,3,5-triazin-2-yl]amino}methyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]-3-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-4-hydroxynaphthalene-2,7-disulfonate
- Details on test material:
- - Name of test material (as cited in study report): H112339 (reference as sample V723)
- Lot/batch No.: NBY 405/74
- Storage condition of test material: darkness at an ambient temperature
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable.
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- The concentration of V723 in the test solutions was measured at the start (excess solution) and end of the test. At the end of the test one replicate of each test concentration was sampled.
Test solutions
- Details on test solutions:
- A solution of 112 mg V723/litre was prepared by addition of V723 to the dilution medium.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test organism was the freshwater crustacean Daphnia magna Straus, obtained from continuous laboratory cultures.
The stock cultures of Daphnia were maintained in a reconstituted water medium, identical to the test dilution water, at a temperature of 20 ± 2°C, and a photoperiod of 16 hours light: 8 hours dark.
The cultures were fed a defined diet of algae Chlorella vulgaris and yeast.
Culture conditions were such that the Daphnia reproduction was by diploid parthenogenesis.
Daphnia <24 hours old were used for testing.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No post exposure observation
Test conditions
- Hardness:
- Hardness (total as CaCO3) 179 mg/l
- Test temperature:
- The temperatures recorded at 0, 24 and 48 hours were 19.8, 19.5 and 19.8°C respectively
- pH:
- 8.4-8.5
- Dissolved oxygen:
- 9.0 - 9.5 mg/l
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- A solution of 112 mg V723/litre was prepared by addition of V723 to the dilution medium. Information supplied with the test sample V723 indicated that it contained 10.5% of water and test concentrations were adjusted accordingly to account for a nominal test concentration of 100 mg/l.
- Details on test conditions:
- Borosilicate glass beakers were used as test vessels, with four replicates per exposure concentration, each containing 200 ml of test solution.
When the test solutions were at test temperature, five Daphnia were added to each vessel in <2.0 ml of dilution water, giving a total of 20 Daphnia per concentration.
The temperature was maintained at 20 ± 1°C and a photoperiod of 16 hours light: 8 hours dark was provided.
The Daphnia were not fed during the course of the test. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The 24 and 48 hour median effective concentrations of Substance HI 12339 to the freshwater crustacean Daphnia magna (<24 hours old) were determined under static test conditions at a temperature of 20 ± 1°C.
The median effective concentration (EC50) is defined as the concentration, calculated from the data obtained, resulting in immobilisation of 50% of the Daphnia in the time period specified.
The values obtained were:
EC50 (mg/l)
24 hour >100
48 hour >100
The values obtained were based on nominal concentrations of Substance HI 12339.
The overall mean of the measured concentrations of the test material as determined at the start and finish of the test was 90% of the nominal value. - Results with reference substance (positive control):
- Not applicable.
- Reported statistics and error estimates:
- Not reported.
Any other information on results incl. tables
Daphnia response
Time |
Nominal |
Number affected per |
Total |
Total |
% |
|||
(hours) |
concn |
|
replicate |
|
number |
number |
affected |
|
|
(mg/1) |
A |
B |
C |
D |
tested |
affected |
|
24 |
Control |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
112 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
48 |
Control |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
112 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- As no effect was found, the 24 and 48 hr EC50 values were >112 mg V723/1 or >100 mg Substance HI 12339/1.
These values were calculated on the basis of the nominal concentration of V723. - Executive summary:
Study conducted to EU test guidance 84/449/EEC part C2 in compliance with GLP.
No effect was found at a concentration of 100mg/l after 48 hours. EC50> 100mg/l. The substance is not considered to pose a hazard to Daphnia.
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