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EC number: 413-550-5 | CAS number: 142068-96-0 H112339
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 08 March 1993 to 31 March 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to recent EU test guidance in compliance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
- Deviations:
- no
- Principles of method if other than guideline:
- The study was conducted following "Bioaccumulation test of chemical substances in fish body" laid down in "the Test requirement on New chemical substances" (KNHOGTO No.5, YAKUHATSU No.615, 49-KIKYOKtf No.392, dated on 13 Jul 1974), and also "305C, Bioaccumulation : Degree of Bioaccumulation in Fish" in "OECD Guidelines for Testing of chemicals" (dated on 12 May 1981).The study was conducted in accordance with the Principles of Good Laboratory Practice (GLP)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate
- EC Number:
- 413-550-5
- EC Name:
- Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate
- Cas Number:
- 142068-96-0
- Molecular formula:
- Hill formula: C53H28Cl2N14Na8O26S8
- IUPAC Name:
- octasodium 5-[(4-chloro-6-{[4-({[4-chloro-6-({7-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-8-hydroxy-3,6-disulfonatonaphthalen-1-yl}amino)-1,3,5-triazin-2-yl]amino}methyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]-3-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-4-hydroxynaphthalene-2,7-disulfonate
- Details on test material:
- - Name of test material (as cited in study report): H112339
- Physical state: Dark red powder
- Analytical purity: 84.66 wt% (in total)
- Impurities (identity and concentrations): Organic minor components in total 3.64wt%, Sodium Chloride 1.2wt%, Water 10.5 wt%
- Lot/batch No.: ASG 9108127
- Stability under test conditions: confimed at preliminary experiments prior to exposure
- Storage condition of test material: cool and dark place
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable. - Radiolabelling:
- no
Sampling and analysis
- Details on sampling:
- Water samples for analysis were taken at 4 times in total, twice per week from test tank as one sample at each sampling time.
Fish analysis was conducted at Week 2, using 2 fish from test tank. Control fish were analysed when the exposure started and when the study completed by sampling 2 fish for each analysis.
Test solutions
- Vehicle:
- no
- Details on preparation of test solutions, spiked fish food or sediment:
- The required amount of test substance was weighed and dissolved into deionised water to prepare 677 mg/l stock solution. It was then supplied to test tank from 25 litre volume glass tank for stock solution. Based on 48 hours LC 50 data and the sensitivity at the analysis on test substance, the nominal test concentration was 1.69 mg/l. Concurrent blank vessels were used as controls
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Species: Carp fevnrinus carpio)
Supplier: Mango Fishfarm centre (Shiga prefecture)
Fish arrived on 15 Dec 92
Breeding conditions
Duration : After removing any abnormal fish at gross examination, fish were treated in a chemical bath and then retained for 27 days at running flow.
chemical bath : Fish were treated for 24 hours in chemical bath of 50 mg/1 Teramycin (Taito Fizer Co.) and 7 g/1 Sodium Chloride at static flow
Acclimatisation
While fish were acclimatised at acclimatisation tank, any abnormal fish were removed. The rest of fish were retained for 27 days at running flow at 25±2°c. After moved into test vessels, for further 20 days, fish were bred at the same temperature at running flow.
Acclimatisation completed : on 19 Feb 93
Weight average 22.0 gr
Length average 9.7 cm
Fat content : average 3.8%
Diets
Type formulated pellet diets for fish Supplier Nihon Haigo Shiryo Co.
Feeding conditions : Diets equivalent to approximately 2% of fish weight was fed in total, twice per day, half at each time. On the day before the termination, no diets was given to fish.
Study design
- Route of exposure:
- aqueous
- Test type:
- semi-static
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 2 wk
Test conditions
- Hardness:
- 120 mg/l
- Test temperature:
- 25 ±2°C
- pH:
- 7.4 - 7.9
- Dissolved oxygen:
- 0.7mg/l
- TOC:
- DOC as follows:
at exposure started : 7.7 mg/l
at exposure completed : 5.9 mg/l - Salinity:
- Not applicable.
- Details on test conditions:
- Test vessel; 1001 volume glass water vessel
Flow rate: stock solution of test sample was put into test vessels at 2mi/min rate and test water was supplied at 800ml/min at 1155 l/day rate.
Test temperature: 25+2°C
DOC concentration: Test solution: 7.1 - 7.7 mg/1
Control: 7.5 - 7.8 mg/1
Number of fish used: Test level: 5 (at start)
Control: 3 (at start)
Test duration: 2 weeks - Nominal and measured concentrations:
- 1.69 mg/l
- Reference substance (positive control):
- no
- Details on estimation of bioconcentration:
- Details in table form submitted under any other information, results section
Results and discussion
Bioaccumulation factor
- Type:
- BCF
- Value:
- < 3.3 dimensionless
- Basis:
- other: Test solution
- Remarks on result:
- other: No further data available from study submitted
- Remarks:
- Conc.in environment / dose:1.69 mg/l
- Details on kinetic parameters:
- Not measured.
- Metabolites:
- Not measured.
- Results with reference substance (positive control):
- Not applicable.
- Details on results:
- The level of accumulation of test substance in carp was all below 3.3 times.
There was no abnormality in test fish by gross observation.
The mean concentration of test substance in test water has been kept constantly at the nominal levels . - Reported statistics:
- No data
Any other information on results incl. tables
Calculation table for analysis of test water
Sample description |
A |
B |
C |
Standard 1.69 mg/l |
30302 |
|
|
Test water at 4thday |
29184 |
1.63 |
|
Standard 1.69 mg/l |
29059 |
|
|
Test water at 7thday |
26521 |
1.54 |
|
Standard 1.69 mg/l |
30553 |
|
|
Test water at 11thday |
30666 |
1.70 |
|
Standard 1.69 mg/l |
27361 |
|
|
Test water at 14thday |
27716 |
1.71 |
1.64 |
A: Peak area (μV-sec) |
|||
A(std): Standard solution |
A(t): Sample |
||
B: Concentration of test substance in test water (mg/l) |
|||
|
B=PxA(l)/A(std) |
|
|
C: Average concentration of test substance in test water (mg/l) |
|||
|
C=(C(1)...C(n)/n |
|
|
|
n: Number of test water analysis |
|
|
C(1): First analysis of test water |
C(n): Last analysis of test water |
||
P: Concentration of standard solution |
1.69 mg/l |
|
Calculation table for recovery and blank test (Analysis of test fish)
Sample description |
A |
B |
C |
D |
E |
G |
|
Standard 1.69 mg/l |
39793 |
|
|
|
|
|
|
Recovery a |
31845 |
1/150 |
20 |
- |
4060 |
79.9% |
|
Recovery b |
30854 |
1/150 |
20 |
- |
3930 |
77.4% |
|
Standard 1.69 mg/l |
40135 |
|
|
|
|
|
|
Blank a |
n.d. |
1/150 |
20 |
- |
- |
|
|
Blank b |
n.d. |
1/150 |
20 |
- |
- |
|
|
a,b: Individual sample |
|||||||
A: Peak area (μV-sec) |
|||||||
|
A(std): Standard solution |
A(t): Sample |
|
|
|||
B: Ratio of portion used for analysis |
|||||||
C: Final volume (ml) |
|||||||
D: Amount of blank in test fish (μg) |
|||||||
E: Amount of test substance recovered (μg) |
|||||||
|
E = PxA(l)/A(std)x1/BXC-D |
||||||
F: Recovery rate (%) |
|||||||
|
F = E/Qx100 |
|
|
|
|
|
|
P: Concentration of test substance in standard solution (mg/l) |
|||||||
Q: Amount of test substance added (5.08mg) |
|||||||
Calculation table for analysis of test fish
Sample description |
A |
D |
G |
K |
H |
J |
|
Standard 1.69 mg/l |
40845 |
|
|
|
|
|
|
Test fish after 2 weeks a |
n.d. |
1 |
23.3 |
- |
1.64 |
- |
|
Test fish after 2 weeks b |
n.d. |
1 |
26.1 |
- |
1.64 |
- |
|
A: Peak area (μV-sec) |
|||||||
|
A(std): Standard solution |
A(t): Sample |
|
|
|||
B: Ratio of portion used for analysis |
1/150 |
|
|
||||
C: Final volume |
20ml |
|
|
|
|
||
D: Dilution factor |
|||||||
E: Average concentration of blank in analysis of control |
|||||||
F: Recovery rate |
78.6% |
|
|
|
|
||
G: Weight of test fish (g) |
|||||||
K: Concentration of test substance in test fish (μg/g) |
|||||||
|
K=(PxA(t)/A(std)/BxDxC/G-E)/Fx100 |
||||||
H: Average concentration of test substance in test water (mg/l) |
|||||||
J: BCF |
|
|
|
|
|
|
|
|
J=(PxA(t)/A(std)/BxDxC/G-E)/Fx100/H |
||||||
P: Concentration of test substance in standard solution |
1.69 mg/l |
||||||
Calculation table for analysis of test fish (Control)
Sample description |
A |
E |
G |
I |
||||
Standard 1.69 mg/l |
42268 |
|
|
|
||||
Before the initiation of exposure a |
n.d. |
- |
21.7 |
- |
||||
Before the initiation of exposure b |
n.d. |
- |
22.6 |
|
||||
|
|
|
|
|
||||
Standard 1.69 mg/l |
40036 |
|
|
|
||||
After the termination of exposure a |
n.d. |
- |
25.7 |
- |
||||
After the termination of exposure b |
n.d. |
- |
22.8 |
- |
||||
A: Peak area (μV-sec) |
||||||||
|
A(std): Standard solution |
A(t): Sample |
|
|||||
B: Ratio of portion used for analysis |
1/150 |
|
||||||
C: Final volume |
|
|
20ml |
|
||||
E: Amount of blank analysis of control (μg) |
||||||||
|
E=PxA(t)/A(std)x/BxC |
|
|
|||||
G: Weight of test fish (g) |
||||||||
I: Concentration of blank in test fish (μg/g) |
||||||||
|
I=E/G |
|
|
|
||||
P: Concentration of test substance in standard solution |
1.69 mg/l |
|||||||
Applicant's summary and conclusion
- Conclusions:
- The level of accumulation of test substance in carp was all below 3.3 times.
- Executive summary:
The purpose of this study was aimed to investigate the level of bioaccumulation factors of H 112339 in carp.
The level of, accumulation of test substance in carp was all below 3.3 times.
There was no abnormality in test fish by gross observation.
The substance is not considered to have the potential to bioaccumulate on the basis of the study conducted.
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