benzyl(diethylamino)diphenylphosphonium 4-[1,1,1,3,3,3-hexafluoro-2-(4-hydroxyphenyl)propan-2-yl]phenolate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

October 24 - November 25, 2003

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2004

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

82857-68-9

Cas Number:

82857-68-9

IUPAC Name:

82857-68-9

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Identity: GM102E
Batch no.: 102780
Expiration date: December 31, 2008
Purity: 99%
Storage conditions: at room temperature at about 20°C, away from direct sunlight

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

The test organism for the study was Scenedesmus subspicatus CHODAT, Strain no. 86.81 SAG, supplied by the Collection of Algal Cultures. The algae had been grown in the RCC laboratories unde standardized conditions according to the test guidelines.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Test conditions

Test temperature:

23°C

Nominal and measured concentrations:

The following nominal concentrations of GM102E were tested: 0.46, 1, 2.2, 4.6 and 10 mg/L. Additionally, a control was testet in parallel

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.91 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 95%CL :0.35 - 1.4

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 95%CL : 1.7 - 3.7

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

6.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 95%CL : 4.3 - 17.8

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 5

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

2.2 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

biomass

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

1.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95%CL : 1.0 - 2.0

Effect concentrations 7

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95%CL :3.7 - 5.8

Effect concentrations 8

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

13.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95%CL : 9.8 - 22.9

Effect concentrations 9

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 10

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

2.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

The analytically determined test item concentrations in the analyzed test media varied in the range of 87 to 98% of the nominal values. Consequently, the test item was sufficiently stable during the test perido of 72 hours under the conditions of the test, and the reported biological results are based on the nominal concentration of the test item.

Applicant's summary and conclusion

Conclusions:

The test item GM102E had a statistically significant inhibition effect on the growth (biomass and growth rate) of Scenedesmus subspicatus after the exposure period of 72 hours at concentrations of 2.2 mg/L.
The 72hour NOEC was determined to be 1.0 mg/L, since up to this test concentration both the mean biomass and he mean growth rate r of the algae were not statistically significantly lower than in the control.