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Reaction mass of sodium potassium 7-amino-4-hydroxy-3,8-bis(2-{2-sulfonato-4-[2-(sulfonatooxy)ethanesulfonyl]phenyl}diazen-1-yl)naphthalene-2-sulfonate, sodium potassium 7-amino-3-{2-[4-(ethenesulfonyl)-2-sulfonatophenyl]diazen-1-yl}-4-hydroxy-8-(2-{2-sulfonato-4-[2-(sulfonatooxy)ethanesulfonyl]phenyl}diazen-1-yl)naphthalene-2-sulfonate, sodium potassium 7-amino-8-{2-[4-(ethenesulfonyl)-2-sulfonatophenyl]diazen-1-yl}-4-hydroxy-3-(2-{2-sulfonato-4-[2-(sulfonatooxy)ethanesulfonyl]phenyl}diazen-1-yl)naphthalene-2-sulfonate and sodium potassium 7-amino-3,8-bis({2-[4-(ethenesulfonyl)-2-sulfonatophenyl]diazen-1-yl})-4-hydroxynaphthalene-2-sulfonate
EC number: 952-120-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- repetition of the test and different test concentrations
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Swiss GLP
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: TVR50
- Expiration date of the lot/batch: Sep 2004
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/vehicle: > 80 g/1 (in water) - Analytical monitoring:
- yes
- Details on sampling:
- All test medium samples for the analytical determinations were taken from the approximate centre of the test vessels (after mixing the medium) and kept at -18 °C to -25 °C until analysis. The concentrations of the test substance at the beginning and at the end of the test were analysed by Analytical Services for Research TD 2.1, Ciba Specialty Chemicals, CH 4002 Basel. Test concentrations below the EC0 were not analysed.
- Vehicle:
- no
- Details on test solutions:
- The test medium used had the following composition :
NaHCO3 65 mg/l
MgSO4 • 7 H2O 123.3 mg/l
KCl 5.8 mg/l
CaCl2 • 2 H2O 294 mg/l
Na2SiO3 4.3 mg/l
NaNO3 274 µg/l
KH2PO4 143 µg/1
K2HPO4 184 µg/1
The test medium was prepared with bidistilled water. The pH was in a range of 7.6 - 8.0. The test medium was oxygen saturated at the beginning of the test.
Total hardness 196 mg/1 CaCO3
Ca / Mg ratio 4 : 1
Na / K ratio 10 : 1
Alkalinity 0.8 mmol/1 - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Specicies : Daphnia magna Straus.
Bred in the laboratories of the Ecotox Center under standardised conditions.
Breeding: Cultures of Daphnia magna are held in glass vessels containing approx. 3.0 litre of reconstituted water at 18 - 22°C. The water is renewed partially three times per week. The Daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a Tetramin-extract in such quantities that the food is consumed within 24 h.
Pre-treatment: 24 h before the start of the exposure reproductive Daphnia were separated from the young Daphnia by sieving all individuals through a 1000 urn sieve.
For each test concentration 20 Daphnia divided into two groups of 10 animals were used. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 196 mg CaCO3/L
- Test temperature:
- 19 - 21 °C
- pH:
- 7.6 - 8
- Dissolved oxygen:
- 95% of maximum
- Nominal and measured concentrations:
- Nominal concentrations : 4.3, 9.4, 21, 45 and 100 mg test substance/l
During the test the actual test concentration in the analysed test media was in the range of 97.9 - 98.2% of the nominal value. - Details on test conditions:
- 14 h light, 10 h dark
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- Reference control October 28, 1998: 24h EC50 1.0 mg/1.
- Reported statistics and error estimates:
- In lack of effects no statistical evaluation was applied.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The determined EC50 (daphnia magna, immobilisation, 48h) is > 100 mg/l as no immobilisation was oibserved at all concentrations tested.
- Executive summary:
Acute toxicity of FAT40571/A to Daphnia magna was carried out according to OECD 202 and EU C.2 guideline to determine the EC50 (concentration at which 50% of the Daphnia population is immobilised).
Total 20 Daphnia at each test concentration (4.3, 9.4, 21, 45 and 100 mg/l nominal) for 48 h duration using Spectrophotometry analytical method.
During the test the actual test concentrations in the analysed test media were in the range of 97.9 - 98.2% of the nominal values. The results are related to the nominal values of the test substance concentrations.
The 24 h and 48 h EC50 is greater than 100mg/l
Reference
During the test the actual test concentration in the analysed test media was in the range of 97.9 - 98.2% of the nominal value. Therefore, the results are related to the nominal value of the test substance concentration.
No immobilisation and abnormal behaviour of the Daphnia was observed in the nominal test concentration of 100 mg/l after the test period of 48 h. Therefore, the EC0value after 48 h was determined to be 100 mg/l. The EC50value after 48 h was determined to be >100 mg/l.
Description of key information
The test substance did not show toxicity in daphnids, the 24 h and 48 h EC50 is greater than 100 mg/l
Key value for chemical safety assessment
Additional information
The acute toxicity to Daphnia magna was carried out according toOECD 202 and EU C.2 guideline to determine the EC50 (concentration at which 50% of the Daphnia population is immobilised).
A total of 20 Daphnia were used at each test concentration and exposed for 48 h to concentrations of 4.3, 9.4, 21, 45 and 100 mg/l test substance. The test item concentrations in test medium were confirmedusing a spectrophotometry analytical method.
During the test the actual test concentrations in the analysed test media were in the range of 97.9 - 98.2% of the nominal values. The results are related to the nominal values of the test substance concentrations.
In this study, the 24 h and 48 h EC50is greater than 100 mg/l, the NOEC was found to be >= 100 mg/l.
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