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EC number: 600-273-1 | CAS number: 102089-74-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 AUGUST 2016 to 7 SEPTEMBER 2016
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Carbamic acid, N-[(1R)-2-hydroxy-1-phenylethyl]-, 1,1-dimethylethyl ester
- EC Number:
- 600-273-1
- Cas Number:
- 102089-74-7
- Molecular formula:
- C13H19NO3
- IUPAC Name:
- Carbamic acid, N-[(1R)-2-hydroxy-1-phenylethyl]-, 1,1-dimethylethyl ester
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Appearance: Powder
Aqueous solubility: 450 mg/L
Purity: 99.99%
Batch No: LS-53-20150815
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The pre-cultivated algae were observed using microscopic to ensure the algae were in good condition. The biomass of pre-cultivated algae was 168.3x 10(4) cells/mL which counted by cell counting. The algal stock solution (100x10(4) cells/mL) was obtained by diluting 59.4mL of pre-cultivated algae to 100mL using growth medium.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Pseudokirchneriella subcapitata was the recommended species in OECD guideline 201. Purchased from Institute of Hydrobiology, Chinese Academy of Sciences, cultured and maintained in the lab. The sensitivity of the algae was checked periodically by determining the 72h-ErC50 of the reference item (Potassium dichromate) to algae. The ErC50 (72h) of Potassium dichromate to the alga (Pseudokirchneriella subcapitata) (batch No. LZ20160422001 (YJ)) was 1.267mg/L, with 95% confidence interval of 1.193mg/L to 1.350mg/L. It proved that the sensitivity of the alga (Pseudokirchneriella subcapitata) fulfilled the criteria.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- 24h intervals
Test conditions
- Test temperature:
- 23°C ± 2°C
- pH:
- 7.8 - 9.2
- Nominal and measured concentrations:
- The test concentration of 25mg/L, 50mg/L, I 00mg/L, 200mg/L and 400mg/L were used in the definitive test based on the result of the preliminary test.The concentrations were in the 80-120% of the nominal.
- Details on test conditions:
- The solution of I 00mg/L, 200mg/L and 400mg/L were prepared as follow: 0.1003g, 0.2002g and 0.400 mg/L of test item were added directly to 1L growth medium, respectively. Then these solutions were mixed at the speed of 600 r/min using stirrers at 25°C for 24 hours. The rest test solutions were obtained by diluting 200mg/L. The algal test solution was obtained by making 0.7mL of algal stock solution (10x10'4 cells/mL) up to 100mL using test solution. Blank control was run simultaneously using growth medium. The control had six replicates and each concentration group had three. Each replicate had 100mL test solution. The measured initial cell concentrations in the test solution and control were approximately 0.8146x 10'4 cells/mL. The test was carried out in the full-temperature light shaking incubator. Spectrophotometer was used in this test to determine the algal biomass. The algal biomass in each flask was determined daily during the test period. Microscopic observation was performed to verify a normal and healthy appearance of the inoculum culture and to observe any abnormal appearance of the algal at the end of the test. Measure the pH of the solution at the beginning and at the end of the test. Concentrations of the test item were analyzed daily during the test.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 348.582 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The growth inhibition concentration was determined by probit using SPSS (Version.17.0). The result showed that the 72h-ErCs of N-Boe-D-phenylglycinol was 348.582mg/L, with the 95% confidence limits range from 286.192mg/L to 458.411mg/L. For estimation of the NOEC, for the effects of the test item on growth rate, the mean for each concentration was compared with the control mean using Dunnett's test by SPSS (Version.17.0). The NOEC ofN-Boc-D-phenylglycinol was 50mg/L.
- Executive summary:
The growth inhibition test of N-Boc-D-phenylglycinol to alga (Pseudokirchneriella subcapitata) was performed in this test. According to the result of the preliminary test, the concentrations of definitive test were 25mg/L, 50mg/L, 100mg/L, 200mg/L and 400mg/L. The solutions of 100mg/L, 200mg/L and 400mg/L were prepared as follow: 0.1003g, 0.2002g and 0.400 mg/L of test item were added to 1L growth medium, respectively. Then these solutions were mixed at the speed of 600 r/min using stirrers at 25C for 24 hours. The 25mg/L and 50mg/L test solutions were obtained by diluting prepared 200mg/L test solution. The algal test solution was obtained by making 0.7mL of algal stock solution (10010' cells/mL) up to I00mL using test solution. Blank control was run simultaneously using growth medium. The control had six replicates and each concentration group had three. Each replicate had 100mL test solution. The initial cell concentrations in the test solution and control were approximately 0.8146x10'4 cells/mL (measured value). All test vessels were placed in the full-temperature light shaking incubator and then the incubation began.The test was carried out in the full-temperature light shaking incubator. Spectrophotometer was used in this test to determine the algal biomass. The algal biomass in each flask was determined daily during the test period. Microscopic observation was performed to verify a normal and healthy appearance of the inoculum culture and to observe any abnormal appearance of the algal at the end of the test. Measure the pH of the solution at the beginning and at the end of the test. Concentrations of the test item were analyzed daily during the test. According to the results, the concentration of the test item was remained within the range 80%-120% of nominal. so analysis of the results was based on nominal concentrations. The 72h-ErC50 of N-Boc-D-phenylglycinol is 348.582mg/L and the 95% confidence limits range from 286.192mg/L to 458.411 mg/L. The NOEC value is 50mg/L.
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