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EC number: 600-273-1 | CAS number: 102089-74-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 20, 2016 to Dec 6, 2016
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- In range-finding test, the concentrations were chosen to be 1.00, 10.0, 100 and 150 mg/L which deviated from protocol the concentrations of 1.00, 10.0 and 100 mg/L. However, the test results showed that the minimum concentration causing 100% mortality
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- This experiment strictly complied with "The guidelines of chemical testing good laboratory practices (HJ/T 155)'', CMA (China Metrology Accreditation) and CNAS (China National Accreditation Service for Conformity Assessment).
Test material
- Reference substance name:
- Carbamic acid, N-[(1R)-2-hydroxy-1-phenylethyl]-, 1,1-dimethylethyl ester
- EC Number:
- 600-273-1
- Cas Number:
- 102089-74-7
- Molecular formula:
- C13H19NO3
- IUPAC Name:
- Carbamic acid, N-[(1R)-2-hydroxy-1-phenylethyl]-, 1,1-dimethylethyl ester
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Batch No. : LS-53-20150815
Appearance: Powder
Aqueous solubility 0.45 g/L (25°C)
Storage conditions: Room temperature ( 10-30°C)
Purity / Assay: 99.99%
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- The analytical results showed that the concentration of the test substance is consistent in the test medium throughout the 96-hour test period (deviation within 20%), thus a static procedure was reasonable.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- In the test, test solutions of N-Boc-D-phenylglycinol had been prepared by directly
dissolving appropriate amount of test substance in dilution medium (Vol: 3L) and then facilitating its
dispersion by stirring for 30 min.
Test organisms
- Details on test organisms:
- Fish (Gobiocypris rarus)
TEST ORGANISM
- Common name: Rare minnow, Gobiocypris rarus
- Strain: (Batch No. F20160415G)
- Source: Obtained from a fish supplier of Institute of Hydrobiology, Chinese Academy of Sciences.
- Length at study initiation (length definition, mean, range and SD): Mean: 2.78cm. Range:2.69-2.92cm.
- Weight at study initiation (mean and range, SD): Mean: 0.222g Range: 0.202-0.35g
ACCLIMATION
- Acclimation period: Fish were held at least for 12 days in holding tanks supplied with a continuous flow of aerated water before being used for testing. Fish to be used in the test were held for at least 7 days in water of the quality and temperature to be used in the test.
A photoperiod of 16 hours light, provided by overhead fluorescent tubes, and 8 hours dark
was maintained. The oxygen concentration was more than 80% of the air saturation value.
FEEDING DURING TEST
- Food type: The ingredients of the fish food are given as follows:
Crude Protein> 36.0%
Crude .Fat> 2.0%
Crude Fibre< 3.0%
Crude Ash <13.5%
Moisture< 10.0%
- Frequency: The fish was fed daily during the holding period on proprietary fish food. They were held without food for approximately 24 hours before being placed into the test vessels.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- The test fishes were exposed for 96 hours to the test solution. The study comprised at least one range-finding test followed by a definitive test to determine the 96-hour median lethal concentration (LC50) and 95% confidence limit of the test substance.
Test conditions
- Hardness:
- The total hardness was in the range of 157 to 168 mg (CaC03)/L.
- Test temperature:
- The temperature of the test mediums was maintained in the range of 22.8°C to 23.2°C
- pH:
- During the whole test period, the pH values of the control mediums and test mediums were
between 7.76 and 7.99. - Dissolved oxygen:
- The Dissolved Oxygen (DO) values varied from 74% ~ 95% of the air saturation.
- Nominal and measured concentrations:
- Nominal concentrations of 1.00, 10.0, 100 and 150 mg/L were used in the range-finding test. While the nominal concentrations of 10.0, 20.0, 40.0, 80.0 and 150 mg/L were used in the definitive test.
For the definitive test, the measured concentrations were 9.10, 20.0, 39.5, 79.6 and 145 mg/L, respectively. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate, K2Cr20 7, CAS: 7778-50-9; Purity: 2: 99.8% Lot number: LVC00524; Beijing Leon Technology Co. Ltd.
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 72.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results showed that under valid static test conditions, the 96 h-LC50 of the test
substance to fish (Rare minnow, Gobiocypris rarus) was 72.4 mg/L, with 95% confidence
limit of 56.8 mg/L - 92.4 mg/L (based on the measured concentration). The maximum
tested concentration causing no mortality (96 h-LC0) was 20.0 mg/L (measured
concentration 20.0 mg/L). The minimum concentration causing 100% mortality (96 h-LC100) was 150 mg/L (measured concentration 145 mg/L), i.e.:
96 h-LC50= 72.4 mg/L, 95% CI 56.8 mg/L - 92.4 mg/L (based on measured concentration);
96 h-LC0 = 20.0 mg/L (measured concentration 20.0 mg/L);
96 h-LC100= 150 mg/L (measured concentration 145 mg/L). - Executive summary:
Under static conditions, the acute toxicity of test substance (N-Boc-D-phenylglycinol) to Rare minnow (Gobiocypris rarus) was conducted according to: "The guidelines for the testing of chemicals" (HJ/T 153-2004), The Guidelines for the Testing of Chemicals, Effects on Biotoc Systems"(the 2nd edition); and with reference to Procedure 203 of the "Guidelines for Testing of Chemicals" of the OECD: "Fish, Acute Toxicity Test" (1992). A range-finding test and then a definitive test were performed respectively. Nominal concentrations of 1.00, 10.0, 100 and 150 mg/L were used in the range-finding test. While the nominal concentrations of 10.0, 20.0, 40.0, 80.0 and 150 mg/L were used in the definitive test. Water samples taken from the blank control and the treatments in the definitive test were analysed. Concentrations of the test substance were quantified using
UPLC-PDA of the measured concentrations were 9.10, 20.0, 39.5, 79.6 and 145 mg/L, respectively. The analytical results showed that the concentration of the test substance is consistent in the test medium throughout the 96-hour test period (deviation within 20% ). Thus a static procedure was reasonable. In the range-finding test, 5 fishes per treatment and no replicates were used. And 10 fishes per treatment of no replicates were used in the definitive test. The test fishes were exposed for 96 hours to the test solution. All validity criteria were met, therefore the test was considered valid.
During the test period, all fish in the control and treatment of 10.0, 20.0 mg/L were alive and appeared normal. Effects (Fish lying on side or back) occurred at concentrations of 40.0 mg/Land above. All Fish were dead after 96 h-exposure at concentration of 150 mg/L. The results showed that under valid static test conditions, the 96 h-LC50 of the test substance to fish (Rare minnow, Gobiocypris rarus) was 72.4 mg/L, with 95% confidence limit of 56.8 mg/L ~
92.4 mg/L (based on the measured concentration). The maximum
tested concentration causing no mortality (96 h-LC0) was 20.0 mg/L (measured
concentration 20.0 mg/L). The minimum concentration causing 100% mortality (96h-LC100) is 150 mg/L (measured concentration 145 mg/L), i.e.:
96 h-LC50 = 72.4 mg/L, 95% CI 56.8 mg/L ~ 92.4 mg/L (based on measured concentration); 96 h-LC0 = 20.0 mg/L (measured concentration 20.0 mg/L);
96 h-LC100 = 150 mg/L (measured concentration 145 mg/L).
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