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EC number: 405-520-5 | CAS number: 95235-30-6 D-8; DD-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 1985-03-22 to 1985-06-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
- Version / remarks:
- May 12, 1981
- Deviations:
- not specified
- GLP compliance:
- not specified
- Radiolabelling:
- no
- Details on sampling:
- a) Test fish:
- Fish body: determined, body weight and length;
- Homogenisation (3 x) in 120 mL Acetone/120 mL Chloroform at 15000 rpm/10 min, filtration; discard residue;
- Organic layer was concentrated under reduced pressure; uptake in 80 mL n-Hexane; extraction 3x with 80 mL Acetonitrile;
separate Hexane/Acetonitrile and concentrate Acetonitrile phase under reduced pressure,
clean up by column chromatography (glass column 15 mm x 30 cm, Florisil packaging 6 g, developing solvent 1st fraction chloroform 50 mL; 2nd fraction chloroform:acetone (9:1) 50 mL);
- The test substance was eluted into the 2nd fraction, which was concentrated under reduced pressure, filled up to 30 mL with THF and subjected to HPLC analysis. Recovery rate of the test substance from the fish body was 85.1 %
- fill up by Tetrahydrofuran (30 mL);
- HPLC determination;
b) Test water:
- Sampling: 150 mL for the 1st concentration group; 750 mL for the 2nd concentration group;
- Extraction 3x with Chloroform: 100 mL for the 1st group; 150 mL for the 2nd group;
- Aqueous layer: discard;
- Chloroform layer: Concentrated under reduced pressure; filled up by tetrahydrofurane (30 mL);
- HPLC determination (20 mL for the 1st group; 10 mL for the 2nd group);
- Recovery rates from test water were 96.0 and 96.5 % for the 1st and 2nd concentration groups, respectively. - Vehicle:
- no
- Details on preparation of test solutions, spiked fish food or sediment:
- Dispersant and dispersing method:
Dispersant: Hardened castor oil (HCO-40);
Dispersing method: The castor oil (HCO-40, 7 g) was melted and dissolved in 20 mL of Acetone with stirring. The test substance of 1.00 g was weighed precisely, added to the acetone solution and dissolved completely. The volume of the solution was filled up to 1.000 mL with distilled water to make the stock solution of 1.000 mg/L. - Test organisms (species):
- Cyprinus sp.
- Details on test organisms:
- TEST ORGANISM
- Common name: carp;
- Strain: not indicated;
- Source: not indicated;
- Age at study initiation: not indicated;
- Length at study initiation: average 10.5 ± 0.4 cm;
- Weight at study initiation: 23.4 ± 3.1 g;
- Weight at termination: not indicated;
- Method of breeding: not indicated;
- Health status: External disinfection by static treatment with 50 mg/L potassium permanganate for 1 h, followed by twice treatment by 50 mg/L Terramycin for 24 h,
- Description of housing/holding area: Test water tank "Flow System", made of glass; volume 100 L; Water flow volume: 300 mL/min (432 L/day);
ACCLIMATION
- Acclimation period: 20 days at 25 °C;
- Acclimation conditions (same as test or not): yes; - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 56 d
- Test temperature:
- 24.0 ± 0.8 °C
- pH:
- 7.5 - 7.7
- Dissolved oxygen:
- 6.6 - 7.7 mg/L
- Nominal and measured concentrations:
- Nominal concentrations:
1st concentration: Test substance: 0.2 mg/L (w/v); dispersant (HCO-40): 1.4 mg/L (w/v);
2nd concentration: Test substance: 0.02 mg/L (w/v); dispersant (HCO-40): 0.14 mg/L (w/v);
Actually determined:
Test groups: 1 W / 2 W / 4 W / 6 W / 8 W;
1st concentration: 0.17 / 0.18 / 0.18 / 0.18 / 0.17 mg/L (w/v);
2nd concentration: 0.016 / 0.016 / 0.017 / 0.018 / 0.017 mg/L (w/v); - Lipid content:
- 4.2 %
- Time point:
- start of exposure
- Remarks on result:
- other: Lipid content was determined by chloroform-methanol extraction (CBC) method.
- Key result
- Type:
- BCF
- Value:
- > 27 - < 78
- Basis:
- whole body w.w.
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc. in environment / dose: 0.2 mg/L
- Key result
- Type:
- BCF
- Value:
- > 40 - < 132
- Basis:
- whole body w.w.
- Time of plateau:
- 42 d
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc. in environment / dose: 0.02 mg/L
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The BCF for D-8 at a concentration level of 0.2 mg/L were determined to be 27 - 78 at 28 days and at concentration level of 0.02 mg/L to be 40 - 132 at 42 days, respectively.
- Executive summary:
D-8 was tested for bioaccumulation in fish in a method similar to OECD Guideline 305C: " Bioaccumulation: Degree of Bioconcentration in Fish", May 1981. D-8 was tested in the concentrations of 0.2 mg test item/L and 0.02 mg test item/L in a continuous flow-through system. The determination of the test item was performed by HPLC analysis. In the first concentration group of 0.2 mg test item/L, bioconcentration factors BCFs of 27 to 78 with a plateau time of 28 days were detected. In the second concentration group of 0.02 mg test item/L, BCFs of 40 to 132 with a plateau time of 42 days could be detected.
Reference
Test Results
Elapsed days |
1st Concentration group: Bioaccumulation factor |
2nd Concentration group: Bioaccumulation factor |
||
A |
B |
A’ |
B’ |
|
3 |
27 |
35 |
40 |
51 |
7 |
29 |
61 |
40 |
63 |
14 |
36 |
70 |
62 |
91 |
21 |
58 |
78 |
90 |
86 |
28 |
57 |
50 |
86 |
109 |
42 |
39 |
36 |
132 |
78 |
56 |
40 |
41 |
84 |
129 |
(Note) The marks A, B, A’, B’ mean the ID numbers of the test fish, given at the sampling times.
Recovery of the test item from the test water was between 94.4 and 97.7 %. A recovery test from the fish body was performed with 4 fish. The recovery was determined to be between 80.5 and 88.9 %.
Description of key information
The BCF for D-8 at a concentration level of 0.2 mg/L were determined to be 27 - 78 at 28 days and at concentration level of 0.02 mg/L to be 40 - 132 at 42 days, respectively. The BCF of 132 was chosen as a keay value for further risk assessment. This values is seen as the worst case.
Key value for chemical safety assessment
- BCF (aquatic species):
- 132 dimensionless
Additional information
D-8 was tested for bioaccumulation in fish in a method similar to OECD Guideline 305C: " Bioaccumulation: Degree of Bioconcentration in Fish", May 1981. D-8 was tested in the concentrations of 0.2 mg test item/L and 0.02 mg test item/L in a continuous flow-through system. The determination of the test item was performed by HPLC analysis. In the first concentration group of 0.2 mg test item/L, bioconcentration factors BCFs of 27 to 78 with a plateau time of 28 days were detected. In the second concentration group of 0.02 mg test item/L, BCFs of 40 to 132 with a plateau time of 42 days could be detected.
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