Quaternary ammonium compounds, C12-14 (even numbered) alkyl(hydroxyethyl)dimethyl, C12 alkylbis(hydroxyethyl)methyl, chlorides and amines, C12-14 (even numbered) alkyldimethyl, chlorides

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 July 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

pycnometer method

Key result

Type:

relative density

Density:

ca. 0.989 other: dimentionless

Temp.:

20 °C

Results

Pre-test for viscosity

The test substance was flowable and it was possible to inject the test substance with a one way syringe.

Measurement

All measured values are given in the following table:

 

Measured Values

First Determination
Weighing #	m1	m2	m3
Substance	Pycn. empty	Pycn. +H2O	Pycn. + test substance
Mass in g	36.7298	61.6084	61.3714
Temperature in^°C	20.2	20.4	19.8
Second Determination
Weighing #	m1	m2	m3
Substance	Pycn. empty	Pycn. +H2O	Pycn. + test substance
Mass in g	36.7300	61.6081	61.3731
Temperature in^°C	19.8	20.0	20.0

Pycn. = Pycnometer

The following values were calculated:

 

Calculated Values

First Determination			Error rel.	Error abs.
Temperature	20.4	°C		0.1
Density of water	0.9982098	g/cm^3	2.00%	0.0002
Volume of Pycnometer	24.9260	cm^3
Density of Test Substance	0.9886	g/cm3	0.0048%	0.0000
Second Determination			Error rel.	Error abs.
Temperature	20.0	°C		0.1
Density of water	0.998203	g/cm^3	2.00%	0.0002
Volume of Pycnometer	24.9229	cm^3
Density of Test Substance	0.9888	g/cm^3	0.0048%	0.0000

rel. = relative; abs. = absolute

 

Presentation of Results

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables:

 

Results of Single Determinations

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	0.9886	0.0000
2	0.9888	0.0000

 

Results – Mean Values

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	24.92	cm^3	0.0022	0.00%
Density of Test Substance	0.9887	g/cm^3	0.0001	0.01%
Density of Test Substance	988.7	kg/m^3	0.1	0.01%
Relative Density of Test Substance	0.9887		0.0001	0.01%

 

Discussion

The density of the test substance was calculated as 0.9887 ± 0.0001 g/cm³ at 20.0 ± 0.4°C. This value is the mean of two independent determinations ± the standard deviation. Standard deviation shows very good correlation of the two determinations. Therefore, the study is considered valid.

Conclusions:

Under the study conditions, relative density of the test substance was determined to be 0.9887.

Executive summary:

A study was conducted to determine the relative density of the test substance, C12-14 HEDMAC (39.6% active) using pycnometer, according to the OECD Guideline 109 and EU Method A.3, in compliance with GLP. Under the study conditions, relative density of the test substance was determined to be 0.9887 (Muckle, 2019).

Description of key information

The relative density of the test substance (39.6% active) was determined using pycnometer method, according to OECD Guideline 109 and EU Method A.3 (Muckle, 2019).

Key value for chemical safety assessment

Relative density at 20C:

0.989