3-pyridyl dimethylcarbamate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-02-23 to 1999-07-08

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Version / remarks:

adopted on 17 July, 1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-pyridyl dimethylcarbamate

EC Number:

257-297-7

EC Name:

3-pyridyl dimethylcarbamate

Cas Number:

51581-32-9

Molecular formula:

C8H10N2O2

IUPAC Name:

pyridin-3-yl N,N-dimethylcarbamate

Specific details on test material used for the study:

- CAS No. : 51581-32-9
- Batch No.: TMFP 304
- Purity: At least 99 %
- Solubility in water: soluble
- Storage: in the refrigerator, in the dark
- Date of expiry: December 1999
- Appearance: Colorless liquid
pH: 7.29

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: J. Schitkovits, A-2620 Natschbach
- Weight (at study initiation and termination) : Animal no. 1: 2.9 kg and 3.1 kg, Animal no. 2: 2.7 kg and 2.9 kg, Animal 3: 2.8 kg and 2.8 kg
- Housing: Individual caging in metal wire cages
- Diet (e.g. ad libitum): Yes
- Water (e.g. ad libitum): Yes
- Acclimation period: 6 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20
- Humidity (%): 53
- Air changes (per hr): 12
- Photoperiod (hrs dark / hrs light): 12 / 12

Test system

Type of coverage:

semiocclusive

Preparation of test site:

clipped

Vehicle:

unchanged (no vehicle)

Controls:

not specified

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL

Duration of treatment / exposure:

4 h

Observation period:

1, 24, 48 and 72 h after patch removal

Number of animals:

3

Details on study design:

TEST SITE
- Area of exposure: 2.5 cm X 2.5 cm

OBSERVATION TIME POINTS : 1, 24, 48 and 72 h
(indicate if minutes, hours or days)

SCORING SYSTEM:
- Method of calculation: Scoring terms of 24, 48 and 72 h after the patch removal were used

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal: 1, 2 and 3

Time point:

24/48/72 h

Score:

0

Results 2

Irritation parameter:

edema score

Basis:

animal: 1, 2 and 3

Time point:

24/48/72 h

Score:

0

Irritant / corrosive response data:

All areas treated with the test substance and all control areas were normal before the application and at each observation time

Other effects:

- Other adverse local effects: None
- Other adverse systemic effects: None

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

In this study, the test substance is considered non-corrosive or a non-irritant to the skin.

Executive summary:

In an in vivo skin irritation study conducted according to the OECD guideline 404, 0.5 mL of the test item was spread on cellulose patches in a size of about 2.5 cm X 2.5 cm and were applied to test sites on each of 3 New Zealand White rabbits for 4 hours. The patches were then removed and the skin was examined for erythema / eschar and oedema as well as other local signs approximately 1, 24, 48 and 72 hours after patch removal. No general toxic effects were observed. All areas treated with test substance and all control areas were normal before the application and at each observation time. Based on these results, the test substance is considered as a non-irritant to the skin under the UN GHS Criteria.