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EC number: 258-203-7 | CAS number: 52821-24-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity Tests: 111. Results from the Testing of 255 Chemicals
- Author:
- Errol Zeiger, Beth Anderson, Steve Haworth, Timothy Lawlor, Kristien Mortelmans, and William Speck
- Year:
- 1 987
- Bibliographic source:
- Environmental Mutagenesis Volume 9, Supplement 9:l-110,1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of Brucin in Salmonella typhimurium strains TA98, TA100,TA1535, and TA1537.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Brucine
- Cas Number:
- 357-57-3
- Molecular formula:
- C23H26N2O4
- IUPAC Name:
- Brucine
- Details on test material:
- Details on test material
- Name of test material (as cited in study report): Brucin
- Molecular formula: C23H26N2O4
- Molecular weight: 394.4684g/mol
- Substance type: Organic
Constituent 1
- Specific details on test material used for the study:
- Details on test material
- Name of test material (as cited in study report): Brucin
- Molecular formula: C23H26N2O4
- Molecular weight: 394.4684g/mol
- Substance type: Organic
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: strains TA98, TA100, TA1535, and TA1537
- Details on mammalian cell type (if applicable):
- Not applicable.
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- The S-9 fractions of Aroclor 1254-induced, male Sprague-Dawley rat and male Syrian hamster livers were prepared
- Test concentrations with justification for top dose:
- 0-6666 µg/plate
- Vehicle / solvent:
- Vehicle
- Vehicle(s)/solvent(s) used: 95% ETHANOL
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- 95% ETHANOL
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- other: -S9 9-aminoacridine (TA97 and TA 1S37), and 4-nitro-o-phenylenediamine (TA98). +S9 2-aminoanthracene for all strains.
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: Preincubation assay - Rationale for test conditions:
- Not specified.
- Evaluation criteria:
- A chemical was judged to be mutagenic (+), or weakly mutagenic (+W), if it
produced a reproducible, dose-related increase in his+ revertants over the corresponding solvent controls in replicate trials - Statistics:
- Standard deviation was observed.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: strains TA98, TA100, TA1535, and TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- Although there were no specific response ranges established for the solvent and positive controls, each laboratory rejected experiments in which the positive
control chemical did not produce a mutagenic response or in which the solvent control values were higher (or lower in the case of TAl00 and TA97) than their expected values. - Remarks on result:
- other: No mutagenic effect were observed
Any other information on results incl. tables
BRUClNE effect on different strain of salmonella
|
TA 100 |
|||||
Dose |
NA (-) |
10% HLI (-) |
10% W (-) |
|||
|
Mean |
Sem |
Mean |
Sem |
Mean |
Sem |
0.000
|
89 |
5.9 |
145 |
13.2 |
159 |
13.2 |
3.000
|
|
|
|
|
|
|
10.000
|
92 |
7.3 |
|
|
|
|
33.000
|
91 |
3.5 |
277 |
10.3 |
284 |
7.8 |
100-000
|
106 |
7.1 |
418 |
30.6 |
409 |
17.7 |
166.000
|
|
|
|
|
523 |
69.4
|
333.000
|
113 |
6.2 |
769 |
91.6 |
792 |
19.8 |
666.000
|
107 |
8.8 |
|
|
|
|
1000.000
|
|
|
1143 |
203.3 |
1200 |
26.5 |
1666.000
|
|
|
|
|
|
|
2000.000 |
|
|
1445 |
36.2 |
|
|
POS
|
361 |
20.4 |
1490 |
135.4 |
586 |
21.1 |
|
TA 1535 |
|||||
Dose |
NA (-) |
10% HLI (-) |
10% W (-) |
|||
|
Mean |
Sem |
Mean |
Sem |
Mean |
Sem |
0.000
|
29 |
3.5 |
14 |
3.8 |
9 |
1 . 5 |
3.000
|
|
|
28 |
5.3 |
15 |
0.6 |
10.000
|
30 |
1.7 |
40 |
2.5 |
26 |
4.2 |
33.000
|
33 |
7.7 |
135 |
4.3 |
85 |
2.6 |
100.000
|
40 |
2.2 |
328 |
5.5 |
227 |
6.6 |
166.000
|
54 |
5.0 |
|
|
|
|
333.000
|
64 |
2.7 |
808 |
15.1 |
617 |
6.8 |
666.000
|
|
|
|
|
|
|
1000.000
|
|
|
|
|
|
|
1666.000
|
|
|
|
|
|
|
2000.000 |
|
|
|
|
|
|
POS
|
331 |
14.6 |
484 |
23.3 |
133 |
8.5 |
|
TA 97 |
|||||
Dose |
NA (-) |
10% HLI (-) |
10% W (-) |
|||
|
Mean |
Sem |
Mean |
Sem |
Mean |
Sem |
0.000
|
98 |
4.3 |
171 |
2.5 |
157 |
9.5 |
3.000
|
106 |
6.4 |
|
|
|
|
10.000
|
112 |
1.2 |
180 |
11.6 |
150 |
12.5 |
33.000
|
96 |
4.3 |
183 |
9.0 |
157 |
8.1 |
100.000
|
63 |
4.9 |
186 |
0.3 |
172 |
8.7 |
166.000
|
|
|
|
|
|
|
333.000
|
35s |
3.6 |
|
|
|
|
666.000
|
|
|
|
|
|
|
1000.000
|
|
|
176 |
6.4 |
156 |
14.6 |
1666.000
|
|
|
|
|
53s |
6.3 |
2000.000 |
|
|
182 |
3.1 |
|
|
POS
|
11353 |
56.7 |
1730 |
34.3 |
884 |
24.2 |
|
TA 1535 |
|||||
Dose |
NA (-) |
10% HLI (-) |
10% W (-) |
|||
|
Mean |
Sem |
Mean |
Sem |
Mean |
Sem |
0.000
|
17 |
1.0 |
26 |
4.8 |
29 |
3.2 |
3.000
|
14 |
0.9 |
|
|
|
|
10.000
|
13 |
0.9 |
39 |
5.9 |
22 |
1.5 |
33.000
|
10 |
1.0 |
32 |
2.3 |
22 |
2.1 |
100.000
|
6 |
0.3 |
34 |
4.6 |
I8 |
1.2 |
166.000
|
0s |
00 |
|
|
|
|
333.000
|
|
|
25 |
6.7 |
13 |
3.5 |
666.000
|
|
|
|
|
0s |
00 |
1000.000
|
|
|
22 |
2.8 |
|
|
1666.000
|
|
|
|
|
|
|
2000.000 |
|
|
|
|
|
|
POS
|
1461 |
22.3 |
993 |
39.6 |
315 |
4.7 |
Applicant's summary and conclusion
- Conclusions:
- Brucin (357-57-3) was evaluated for its mutagenic potential in Salmonella typhimurium strains TA98, TA100, TA1535, and TA1537 by Bacterial reverse mutation Assay. The test result was considered to negative in the presence and absence of S9.
- Executive summary:
Brucin was assessed for its possible mutagenic potential. For this purposeBacterial reverse mutation Assay was performed on Salmonella typhimurium strains TA98, TA100, TA1535, and TA1537 .The test material was exposed at the concentration of 0-6666 µg/plate in the presence and absence of S9. No mutagenic effects were observed in all strain. Therefore Brucin was considered to be non mutagenic inSalmonella typhimurium strains TA98, TA100, TA1535, and TA1537. Hence the substance cannot be classified as gene mutant in vitro.
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