Registration Dossier
Registration Dossier
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EC number: 274-159-1 | CAS number: 69852-45-5
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: A method for determining the dermal toxicity of pesticides. British Journal of Industrial Medicine, Vol 26, page 59-64, 1969
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 3-[[3-(dimethylamino)propyl]amino]propiononitrile
- EC Number:
- 274-159-1
- EC Name:
- 3-[[3-(dimethylamino)propyl]amino]propiononitrile
- Cas Number:
- 69852-45-5
- Molecular formula:
- C8H17N3
- IUPAC Name:
- 3-{[3-(dimethylamino)propyl]amino}propanenitrile
- Reference substance name:
- 3,3'-[[3-(dimethylamino)propyl]imino]bispropiononitrile
- EC Number:
- 275-331-9
- EC Name:
- 3,3'-[[3-(dimethylamino)propyl]imino]bispropiononitrile
- Cas Number:
- 71326-27-7
- Molecular formula:
- C11H20N4
- IUPAC Name:
- 3,3'-{[3-(dimethylamino)propyl]imino}dipropanenitrile
- Test material form:
- liquid
- Remarks:
- colorless
Constituent 1
impurity 1
- Specific details on test material used for the study:
- Batch number Sdg. 807701
Test animals
- Species:
- rabbit
- Strain:
- other: Tvanovas, 7964 Kisslegg/Allggsu
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 2 to 3 kg
- Housing: Wire cages, 10 hours a day light cycle
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: minimum of 4 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2°C
- Humidity (%): 55 +/- 10%
- Photoperiod (hrs dark / hrs light): 14 hrs dark / 10 hrs light
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Type of wrap if used: Adhesive, elastic
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Washed with lukewarm water
- Time after start of exposure: 24 hours after treatment
TEST MATERIAL
- Concentration (if solution): 1, 2, 4 and 6 ml/kg body weight
- Duration of exposure:
- 24 hours
- Doses:
- 1, 2, 4 and 6 ml/kg body weight
1000, 2000, 4000 and 6000 mg/kg - No. of animals per sex per dose:
- 3
- Control animals:
- yes, concurrent no treatment
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Day 1, 7 and 14
- Necropsy of survivors performed: yes/no Yes
- Other examinations performed: Sedation, dyspnoea, dacryorrhoea, chromydacryorrhoea, rinorrhea, epistaxis, salivations, ruffled fur, pallor, cyanosis, diarrhoea, body position (ventral), body position (lateral), body position (curved), ataxia, tirmus, tremor, tonic cllonic muscle spasms, convulsions.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 4 500 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 100% at top dose (6000 mg/kg)
- Clinical signs:
- After 24 hours all animals at doses 1000 and 2000 mg/kg showed signs of sedation, ruffled fur and had a curved body position. They recovered from sedation after 11 days, ruffled fur after 8 days and curved body position after 5 days.
At the 4000 mg/kg dose level the same symptoms were seen at 2 hours, along with ataxia and tremor at day 2, which were gone on day4. - Body weight:
- Body weight remained consistent throughout the study
- Gross pathology:
- No substance related gross organ changes were seen.
- Other findings:
- The material was shown to cause severe skin irritation
Applicant's summary and conclusion
- Interpretation of results:
- Category 5 based on GHS criteria
- Conclusions:
- The acute dermal LD50 of TK 12271 in rabbits of both sexes observed over a period of 14 days is approximately 4 500 mg/kg. The test material has therefore a slight acute toxicity to the rabbit by this route of application.*
Severe skin irritation did occur.
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