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Diss Factsheets

Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from secondary source.

Data source

Reference
Reference Type:
secondary source
Title:
Studies on 5,12-dihydro-quino<2,3-b>acridine-7,14-dione with Attachments
Author:
Environmental Protection Agency, Washington, DC. Office of Toxic
Year:
1991
Bibliographic source:
Office of Toxic Substances, EPA/OTS; Doc #86-920000391S, NTIS/OTS0533729, 1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: As mentioned below
Principles of method if other than guideline:
Test for skin sensitization potential have been conducted on 5, 12-dihydroquino[2,3-b]acridine-7,14-dione.
GLP compliance:
not specified
Type of study:
other: No data available.
Justification for non-LLNA method:
Not specified.

Test material

Constituent 1
Chemical structure
Reference substance name:
5,12-dihydroquino[2,3-b]acridine-7,14-dione
EC Number:
213-879-2
EC Name:
5,12-dihydroquino[2,3-b]acridine-7,14-dione
Cas Number:
1047-16-1
Molecular formula:
C20H12N2O2
IUPAC Name:
5,12-dihydroquino[2,3-b]acridine-7,14-dione
Specific details on test material used for the study:
- Name of test material (as cited in study report): quinacridone (5, 12-dihydroquino[2,3-b]acridine-7,14-dione)
- Molecular formula: C20H12N2O2
- Molecular weight: 312.322 g/mol
- Substance type: Organic
- Physical state: Solid

In vivo test system

Test animals

Species:
guinea pig
Strain:
other: albino
Sex:
male
Details on test animals and environmental conditions:
No data available.

Study design: in vivo (non-LLNA)

Induction
Route:
epicutaneous, open
Vehicle:
other: Water containing 1% Duponol PT
Concentration / amount:
10%
Challenge
Route:
epicutaneous, semiocclusive
Vehicle:
other: Water containing 1% Duponol PT
Concentration / amount:
10%
No. of animals per dose:
10 animals.
Details on study design:
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: No data
- Exposure period: no data
- Test groups: yes
- Control group: No data
- Site: intact skin
- Frequency of applications: No data
- Duration: No data
- Concentrations: 10%

B. CHALLENGE EXPOSURE
- No. of exposures: 9
- Day(s) of challenge: 14 days
- Exposure period: 14 days
- Test groups: yes
- Control group: No data
- Site: abraded skin
- Concentrations: 10%
- Evaluation (hr after challenge): 336 hour
Challenge controls:
No data available
Positive control substance(s):
not specified

Study design: in vivo (LLNA)

Statistics:
No data available.

Results and discussion

In vivo (non-LLNA)

Results
Reading:
1st reading
Hours after challenge:
336
Group:
test chemical
Dose level:
10%
No. with + reactions:
0
Total no. in group:
10
Clinical observations:
Produced mild irritation, but showed no sensitization reaction.
Remarks on result:
no indication of skin sensitisation

In vivo (LLNA)

Cellular proliferation data / Observations:
CLINICAL OBSERVATIONS:Produced mild irritation, but showed no sensitization reaction.

Any other information on results incl. tables

No indication of skin sensitization.

Applicant's summary and conclusion

Interpretation of results:
other: not sensitizing
Conclusions:
5,12-dihydroquino[2,3-b]acridine-7,14-dione at a 10% concentration in aqueous suspension was a mild irritant at both intact and abraded skin of albino guinea pigs. However, the 5,12-dihydroquino[2,3-b]acridine-7,14-dione assessed not to be a skin sensitizer in the test with guinea pigs.
Executive summary:

Tests for skin sensitization potential have been conducted for 5, 12-dihydroquino[2,3-b]acridine-7,14-dione (1047-16-1) on albino guinea pigs.When a 10% suspension of the material in water containing 1% of Duponol PT was applied to intact skin of 10 male albino guinea pigs, it produced mild irritation on five animals and none on the others. Applications of the same concentration to abraded skin were slightly more irritating.Challenge tests, following nine exposures of the 10% suspension to abraded skin and 2 weeks rest period, showed no sensitization potential. Therefore 5, 12-dihydroquino[2,3-b]acridine-7,14-dione (1047-16-1) was considered to be non sensitizer in albino guinea pigs.