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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The substance is not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

In a GLP-compliant ready biodegradability test performed according to OECD guideline 301A, degradation of the test substance by micro-organisms was determined.

In this study, the test substance (40 mg/L) was exposed to a mixture of polyvalent bacteria (1,4E+06 cells/mL) from a effluent of a domestic sewage treatment plant (ARA-Rhein) for 28 days. After 28 days no biodegradation was observed.

The biological oxygen demand in 5 days (BOD5) was determined in a study performed according to the EEC guideline C.8 (CG 1987). The testsubstance (1, 5, 10, 20, 50, 100, 200 and 500 mg/L) was exposed to a mixed culture of bacteria (an effluent of a Husman laboratory apparatus). The BOD5 of the test substance, corrected by the blank control was 0 mg/g oxygen. The chemical oxygen demand (COD) for the test substance was determined in a test following the EEC guideline C.9 (CG 1980). The COD was 746 mg O2/g test material. The BOD5/COD ration supports the conclusion that the test substance is not readily biodegradable.