Registration Dossier
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EC number: 203-757-7 | CAS number: 110-33-8
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
No toxic effects up to the limit of water solubility (2.6 mg/L in aqua dest) for Daphnia magna (OECD 202)
Key value for chemical safety assessment
Additional information
One study investigating the acute toxicity to aquatic invertebrates of Dihexyl adipate (CAS 110-33-8) is available. The study was conducted under GLP conditions according to OECD guideline 202 using Daphnia magna as test organism (Harris, 2013). The test organisms were exposed to an aqueous solution of the test item at concentrations of 1.0, 3.2, 10, 32 and 100% v/v saturated solution for 48 hours at a temperature of 20 °C to 21 °C under semi static test conditions. The test item solution was prepared by stirring an excess (50 mg/L) of test item in test medium using a propeller stirrer at approximately 1500 rpm for 24 hours. After the stirring period any undissolved test item was removed by filtration (0.2 µm Gelman Acrocap filter, first approximate 500 mL discarded in order to pre-condition the filter) to produce a 100% v/v saturated solution of the test item from which dilutions were prepared to give the remainder of the test series. The number of immobilized Daphnia was recorded after 24 and 48 hours. Analysis of the freshly prepared test media at 0 and 24 hours showed measured test concentrations of 0.538 and 0.415 mg/L respectively. A decline in measured test concentration was observed in the old or expired test media at 24 and 48 hours to less than the limit of quantitation (LOQ) of the analytical method employed, which was determined to be 0.0022 mg/L, and 0.0111 mg/L respectively. This decline in concentration was considered to be due to the unstable nature of the test item. Given this decline in measured test concentrations over each 24 hour period it was considered justifiable to base the results on the time weighted mean measured test concentrations of the test media to give a "worst case" analysis of the data. The time weighted mean measured test concentration was calculated to be 0.099 mg/L. The 48-Hour EC50 based on the time weighted mean measured test concentrations test media was greater than 0.099 mg/L and correspondingly the No Observed Effect Concentration was 0.099 mg/L.
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