Methyl 3-mercaptopropionate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

N/A to 1983-07-14

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study was conducted with methods similar to OECD 401. However, information on the test substance was lacking (i.e., purity).

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

Information on the test substance (i.e., purity) was lacking.

Principles of method if other than guideline:

N/A

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl 3-mercaptopropionate

EC Number:

220-912-4

EC Name:

Methyl 3-mercaptopropionate

Cas Number:

2935-90-2

Molecular formula:

C4H8O2S

IUPAC Name:

methyl 3-sulfanylpropanoate

Details on test material:

- Name of test material (as cited in study report): methyl 3-mercaptopropionate
- Molecular formula (if other than submission substance): N/A
- Molecular weight (if other than submission substance): N/A
- Smiles notation (if other than submission substance): N/A
- InChl (if other than submission substance): N/A
- Structural formula attached as image file (if other than submission substance): N/A
- Substance type: active
- Physical state: liquid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: N/A
- Age at study initiation: young adult
- Weight at study initiation: 203-288 g
- Fasting period before study: Animals were fasted 18 to 24 hours prior to dosing.
- Housing: Animals were housed two per cage.
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least one week


ENVIRONMENTAL CONDITIONS
- Temperature (deg. C): Every attempt was made to maintain temperatures at 70+/-4 deg. F.
- Humidity (%): Every attempt was made to maintain a relative humidity of 40-60 %.
- Air changes (per hr): N/A
- Photoperiod (hrs dark / hrs light): 12/12


IN-LIFE DATES: From: N/A To: N/A

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

VEHICLE
- Concentration in vehicle: N/A
- Amount of vehicle (if gavage): N/A
- Justification for choice of vehicle: N/A
- Lot/batch no. (if required): N/A
- Purity: N/A


MAXIMUM DOSE VOLUME APPLIED: Dosage volume for each rat was based on initial body weights.


DOSAGE PREPARATION (if unusual): N/A


CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: N/A

Doses:

50, 100, 150, 250, 500 and 5000 mg/kg bw

No. of animals per sex per dose:

5/sex/dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations performed on day of dosing (1, 2 and 4 hours) and twice daily thereafter. Observations included nature, onset, severity and duration of pharmacotoxic signs. Body weights were recorded at study initiation, day 7 and at termination (day 14).
- Necropsy of survivors performed: yes
- Other examinations performed: gross pathology

Statistics:

LD50 calculations were estimated by the Behrens-Reed-Meunch method.

Results and discussion

Preliminary study:

N/A

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

206.4 mg/kg bw

95% CL:

149 - 285.9

Remarks on result:

other: See mortality section below.

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

181.7 mg/kg bw

95% CL:

132.6 - 248.9

Remarks on result:

other: See mortality section below.

Effect levels 3

Sex:

male/female

Dose descriptor:

LD50

Effect level:

193.6 mg/kg bw

95% CL:

154.2 - 243.2

Remarks on result:

other: See mortality section below.

Mortality:

Males:
No animals died in the groups exposed to 50-150 mg/kg of the test substance. 4 animals in the 250 mg/kg dose group died (within 2 hours of treatment) and all of the animals in the 500 and 5000 mg/kg dose groups died (within 1 hour of treatment).

Females:
One animal died (within 1 hour of treatment) in the 150 mg/kg dose group. All animals died (within 2 hours of treatment) in the dose groups of 250-5000 mg/kg.

Clinical signs:

other: Clinical observations were noted among all rats during the study. All rats dosed at 50 mg/kg bw exhibited slight depression on Day 1 post-dose only. Clinical signs noted in animals of remaining dose levels included one or more of the following: depressi

Gross pathology:

There were no gross pathological findings noted in rats surviving to termination. Findings among the rats that were found dead included dark red lungs, test substance-like material in the stomach and/or intestines, and reddish fluid in the intestines.

Other findings:

- Organ weights: N/A
- Histopathology: N/A
- Potential target organs: N/A
- Other observations: N/A

Any other information on results incl. tables

N/A

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Remarks:

Migrated information

Conclusions:

The acute oral LD50 in male and female rats was determined to be 193.6 mg/kg bw.

Executive summary:

N/A