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EC number: 939-719-8 | CAS number: 5502-75-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 2, 2011 to September 6, 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 2010
- Deviations:
- yes
- Remarks:
- The initial and final dissolved oxygen concentrations were outside those recommended in the test guidelines. This was considered to have had no adverse effect on the results of the study.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Inspected on July 19-21, 2011 / Signed on August 31, 2011
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Purity test date: March 21, 2012 - Analytical monitoring:
- not required
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: The activated sewage sludge sample was maintained on continuous aeration in the laboratory at a temperature of approximately 21ºC overnight prior to use in the test. On the day of collection the activated sewage sludge (10 litres) was fed synthetic sewage sludge (500 mL).
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No
- Hardness:
- Not reported
- Test temperature:
- 20±2ºC
- pH:
- the pH of the synthetic sewage stock used to feed the activated sewage sludge was pH 7.1 and the pH of the synthetic sewage stock used for the definitive test was pH 7.8.
- Dissolved oxygen:
- 5.3-7.0 mg O2/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal: 0 (control), 100, 180, 320, 560 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL conical flask
- Aeration: yes
- No. of organisms per vessel: The suspended solids concentration was equal to 3.0 g/L prior to use in the inoculum. 250 ml inoculum was added to each vessel
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised reverse osmosis water
- Total organic carbon: less than 1 mg/L Dissolved Organic Carbon
OTHER TEST CONDITIONS
- Photoperiod: continuous
- Light intensity: normal laboratory lighting
EFFECT PARAMETERS MEASURED: Oxygen Consumption
TEST CONCENTRATIONS
- Range finding study: yes (10, 100 and 1000 mg/L)
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 190 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL=170-210
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- 310 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes (3h EC50 = 8.9 mg/L)
- Validity criteria fulfilled:
- yes
- Remarks:
- In some instances, initial and final dissolved oxygen concentrations were outside those recommended in the test guidelines. This was considered to have had no adverse effect on the results of the study.
- Conclusions:
- The 3 hour EC50 was determined to be 190 mg/L based on respiration rate.
- Executive summary:
A study was performed, according to OECD Guideline 209, to assess the effect of the test item on the respiration of activated sewage sludge. Following a preliminary range-finding test, activated sewage sludge was exposed to an aqueous dispersion of the test item at concentrations of 100, 180, 320, 560 and 1000 mg/L (3 replicates for each test concentration) for a period of 3 hours at a temperature of 20 ± 2 °C with the addition of a synthetic sewage as a respiratory substrate. The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-dichlorophenol. The coefficient of variation of oxygen uptake in the control vessels was 6.85% and the specific respiration rate of the controls was 31.33 mg oxygen per gram dry weight of sludge per hour. The validation criteria have therefore been satisfied. The validation criterion for the reference item EC50 value was also satisfied. In some instances, the initial and final dissolved oxygen concentrations were outside those recommended in the test guidelines (7.0 mg O2/L and 2.0 mg O2/L respectively). This was considered to have had no adverse effect on the results of the study given that in all cases the oxygen consumption rate was determined over the linear portion of the oxygen consumption trace. The dissolved oxygen concentrations in all vessels were above 60 to 70% of the dissolved oxygen saturation level of 8.9 mg O2/L. There was a higher oxygen consumption rate in the 180 mg/L replicate R1 test vessel compared to replicates R2 and R3. This resulted in a lower inhibition value for the 180 mg/L replicate R1 test vessel compared to replicates R2 and R3. However the EC values are calculated based on the mean degradation value for the three replicate vessels for each concentration so it is considered to have no effect on the study.
The effect of the test item on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC50 value of 190 mg/L, 95% confidence limits 170 - 210 mg/L.
Reference
Description of key information
The substance was reported to have low toxicity to microorganisms derived from activated sludge, with a 3-hour EC50 value for growth inhibition of 190 mg/L (nominal) in an OECD guideline study (Test No. 209).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 190 mg/L
Additional information
The inhibitory concentration of the substance on the respiration of aerobic waste water bacteria was determined in a mixed population of activated sewage sludge (Harland Laboratories, 2012). The test method followed the OECD Guidelines for Testing of Chemicals No. 209. 3,5-Dichlorophenol was used as the reference substance at concentrations of 3.2, 10, and 32 mg/L. In this study the oxygen consumption rates were measured after time of 3 hours at nominal concentrations of 100, 180, 320, 560, and 1000 mg/L. The inhibitory concentration (EC50) (3h contact time) for reference substance was 8.9 mg/L, while the EC50 value (3h contact time) for the test substance was 190 mg/L (mean measured), suggesting that the substance is not toxic to microorganisms.
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