Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-106-7 | CAS number: 7439-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: cytogenicity / chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well documented publication with some restriction: material and methods not described in detail
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Effect of chlorophyllin on mercuric chloride-induced clastogenicity in mice.
- Author:
- Ghosh, A.K.; et al.
- Year:
- 1 991
- Bibliographic source:
- Food and Chemical Toxicology, 29(11):777–779.
- Reference Type:
- publication
- Title:
- Mammalian in vivo cytogenetic assay. Analysis of chromosome aberrations in bone marrow cells.
- Author:
- Preston, R.J.; et al.
- Year:
- 1 987
- Bibliographic source:
- Muatation Research 189, 157-165
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: Mamallian in vivo cytogenetic assays. Analysis of chromosome aberrations in bone marrow cells. (Preston, R.J.; et al., 1987, Mutation Research 189: 157-165)
- Deviations:
- not specified
- GLP compliance:
- no
- Type of assay:
- chromosome aberration assay
Test material
- Reference substance name:
- Mercury chloride
- EC Number:
- 231-430-9
- EC Name:
- Mercury chloride
- Cas Number:
- 7546-30-7
- IUPAC Name:
- mercury dichloride
- Reference substance name:
- Mercury dichloride
- EC Number:
- 231-299-8
- EC Name:
- Mercury dichloride
- Cas Number:
- 7487-94-7
- IUPAC Name:
- mercury dichloride
- Details on test material:
- - Name of test material (as cited in study report): mercury chloride
- Molecular formula (if other than submission substance): HgCl2
- Physical state: solid
No further details are given.
Constituent 1
Constituent 2
Test animals
- Species:
- mouse
- Strain:
- Swiss
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Swiss albino inbred mice were obtained from the Departmental animals house.
- Age at study initiation: 8-10 weeks
- Weight at study initiation: 25-28 g
- Housing: in cages
- Diet: standard diet
- Water: ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 28 +/- 2
- Humidity (%): 60 +/- 5
- Photoperiod: 12 hours dark/light cycle
No further details are given.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- Vehicle(s)/solvent(s) used: distilled water
- Details on exposure:
- No further information available.
- Duration of treatment / exposure:
- single treatment
- Frequency of treatment:
- once
- Post exposure period:
- Animals were killed 24 hours after exposure.
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
3.0 mg/kg body weight
Basis:
nominal in water
2.2 mg/kg bw Hg
- Remarks:
- Doses / Concentrations:
6.0 mg/kg body weight
Basis:
nominal in water
4.4 mg/kg bw Hg
- Remarks:
- Doses / Concentrations:
12.0 mg/kg body weight
Basis:
nominal in water
8.9 mg/kg bw Hg
- No. of animals per sex per dose:
- 5 animals were used for each dose.
- Control animals:
- yes
- Positive control(s):
- Cyclophosphamide:
- Justification for choice of positive control(s): according to the followed guideline
- Route of administration: intraperitoneal
- Doses / concentrations: 25 mg/kg body weight
Examinations
- Tissues and cell types examined:
- Bone marrow
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION:
- Doses were equivalent to 1/40, 1/20 and 1/10 of the LD50 determined in the strain of mice used.
TREATMENT AND SAMPLING TIMES ( in addition to information in specific fields): no further details.
DETAILS OF SLIDE PREPARATION:
- Bone marrow chromosomes were prepared following a standard protocol: colchicine (4 mg/kg body weight; i.p.), hypotonic (1% sodium citrate), fixative (1:3 glacial acetic acid/ethanol) and flame drying.
- The slides were stained in diluted Giemsa stain.
METHOD OF ANALYSIS:
- 50 perfect metaphase plates were scored randomly per animal for chromosomal aberrations, and 1000 cells were scored for the mitotic index.
- All aberrations (chromatid gap, chromosome gap, chromatid break, chromosome break and rearrangement) were considered equal, irrespective of the number of events involved.
- The results are expressed as percentages of aberrant metaphase cells excluding gaps (%DC), and numbers of aberrations per cell excluding gaps (CA/cell). - Evaluation criteria:
- no data
- Statistics:
- For all statistical analyses, the level of significance was established at P<0.05. A one-tailed trend test was performed to observe the effects of HgCl2. A one-way ANOVA test followed by Duncan's multiple range test was carried out to detect significant differences among different treatment protocols. Student's t-test was used to compare the mitotic index in the different sets of experiments.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- positive
- Remarks:
- HgCl2 at dose of 3, 6 and 12 mg/kg bw increased the frequency of CA (chromatid and isochromatid gaps, chromatid breaks). The number and percentage of damaged cells as well as the CA per cell were dose-dependently increased.
- Toxicity:
- yes
- Remarks:
- The mitotic indices were significantly and dose related decreased over the dose range investigated.
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- No further details on results are available.
Any other information on results incl. tables
Table: Total chromosomal aberrations recorded following oral treatment of mice with mercury chloride.
Concentration [mg/kg body weight] |
Total chromosomal aberrations |
DC% (mean ± SEM) |
CA/cell (mean ± SEM) |
Mitotic index (mean ± SD) |
|||||
G’ |
G* |
B’ |
B* |
RR |
DC |
||||
0 |
3 |
0 |
2 |
0 |
0 |
2 |
0.80 ± 0.49 |
0.008 ± 0.004 |
4.34 ± 0.11 |
3 |
18 |
1 |
9 |
0 |
1 |
10 |
4.0 ± 0.89 |
0.04 ± 0.008 |
3.78 ± 0.39§* |
6 |
19 |
5 |
12 |
0 |
1 |
13 |
5.2 ± 1.01 |
0.052 ± 0.01 |
2.52 ± 0.19** |
12 |
23 |
2 |
16 |
0 |
2 |
18 |
7.2 ± 0.79 |
0.072 ± 0.007 |
1.9 ± 0.48*** |
Positive control (25 mg/kg bw) |
39 |
2 |
282 |
2 |
15 |
109 |
43.6 ± 9.53 |
1.188 ± 0.343 |
1.76 ± 0.44*** |
G’ and G* = chromatid and isochromatid gaps, respectively; B’ and B* = chromatid and chromosome breaks, respectively; RR = chromosomal arrangement; DC = damaged cells; SEM = standard error of the mean; SD = standard deviation.
§ = significant positive dose response obtained (P<0.001, one-tailed trend test).
* = indicates significant reductions in mitotic index compared with the negative control (* = P<0.05; ** = P<0.01 and *** = P<0.001; Student’s t-test).
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): positive
Under the described test conditions and according to the given information the test substance mercury chloride showed mutagenic activity in the chromosome aberration test at all dose levels investigated. - Executive summary:
To evaluate the potential of mercury chloride to induce chromosome aberration in bone marrow cells, male mice were treated once with three different doses of the substance (per oral). The results showed that HgCl2 at dose levels of 3, 6 and 12 mg/kg bw ( 2.2, 4.4 and 8.9 mg/kg bw/d Hg) increased the frequency of chromosomal aberrations (chromatid and isochromatid gaps, chromatid breaks). The number and percentage of damaged cells as well as the chromosomal aberrations per cell were dose-dependently increased. The mitotic indices were significantly and dose related decreased over the dose range investigated.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.