Cyclohexyl isocyanate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data from peer-reviewed handbook or collection of data

Data source

Reference

Reference Type:

review article or handbook

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexylamine

EC Number:

203-629-0

EC Name:

Cyclohexylamine

Cas Number:

108-91-8

Molecular formula:

C6H13N

IUPAC Name:

cyclohexanamine

Details on test material:

cyclohexylamine, purity not specified

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

concentrations in the test solution was determined in the freshly prepared test solution and after 48 h of exposure

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

20 °C +/- 1 °C

Nominal and measured concentrations:

nominal concentrations: 5.3 mg/L, 9.5 mg/L, 17.1 mg/L, 30.9 mg/L, 55.6 mg/L, 100.0 mg/L
measured concentrations t0: 5.2 mg/L, 9.0 mg/L, 16.3 mg/L, 28.8 mg/L, 54.7 mg/L, 100.9 mg/L
measured concentrations 48 h: 5.3 mg/L, 9.2 mg/L, 16.4 mg/L, 29.5 mg/L, 53.0 mg/L, 99.3 mg/L,

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

36.3 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

other: immobilization

Remarks on result:

other: 95 % CL: 30.4 to 44.0 mg/L

Details on results:

- no mortality in control group after 48 h of exposure
- pH values of individual concentration levels increased form 7.4 (5.3 mg/L) up to 10.1 (100.0 mg/L)
- pH values over time did not vary more than 1.5 units (except samples containin g100 mg/L test item)
- dissolved oxygen exceeds 8.1 mg/L throughout the entiry test duration

Any other information on results incl. tables

- at the highest test concentration of 100 mg/L all test animals have already died after 24 h probably caused by the prevailing pH of 10.1

- measured concentrations always were greater than 80 % of nominal concentrations (the lowest measured concentration accounted for 94.7 % of nominal values) justifying the use of nominal concentration for test evaluation

Applicant's summary and conclusion

Executive summary:

The acute toxicity of cyclohexylamine to aquatic invertebrates (Dapnia magna) was tested according to OECD TG 202. After 48 hours of exposure an EC50 of 36.3 mg/L was obtained (MITI, 1997).