3-Morpholinone, 4-[4-[(5S)-5-(aminomethyl)-2-oxo-3-oxazolidinyl]phenyl]-, hydrochloride (1:1)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Mar 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PRETREATMENT OF TEST ITEM:
- 105.3 mg of the test item added to 1 L of dilution water and treated for 1 h in an ultrasonic bath.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS, parthenogenetic females, strain of Bundesgesundheitsamt Berlin

Maintenance and Acclimatisation:
A population of parthenogenetic females of synchronized age structure has been maintained for more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination > 1000 lux). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily in a semi-quantitative way. The neonates were separated from their parent Daphnia by filtration prior to the acute test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

16.0 °dH (= 286 mg/L CaCO3)

Test temperature:

20.6 °C after 48 h

pH:

7.9 after 48 h

Dissolved oxygen:

8.5 mg/L after 48 h

Nominal and measured concentrations:

100 mg/L (nominal) plus control
Results of analysis of test substance concentration (0 and 100 mg/l) in test solutions at 0 and 48 h showed that the substance stayed stable through the test duration (Measured concentrations were in range of 101.5 - 102.8% of nominal concentrations).

Details on test conditions:

TEST SYSTEM:
- Test vessels: 50 mL glass beakers covered with watch glass holding 20 mL of test solution
- 1 test concentrations (100 mg/L) plus 1 control
- 10 neonates per vessel, 2 replicates per concentration/control
- Temperature of incubation unit : 18-22 °C
- No feeding during the exposure period
- Photoperiod: 16 h light: 8 h dark
- No aeration
- Medium renewal: none
- Criteria of effects: item-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neoates, observed at 24 and 48 h.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Recovery rates correspond to 102.8% of nominal values at 0 hours, and to 101.5% of nominal values at 48 hours, respectively.

Validity criteria fulfilled:

yes

Remarks:

(The immobilisation and other abnormalities in the controls did not exceed 10% by the end of the test. - The dissolved oxygen concentration remained above 3 mg/L throughout the exposure period.)

Conclusions:

EC50 (48 h) > 100 mg/L (nominal)

Executive summary:

In order to test acute toxicity to invertebrates of the substance, Daphnia magna was exposed to a limit concentration of 100 mg/L and a blank control solution for a period of 48 h under static conditions. Mobility and visible abnormalities were recorded at 24 and 48 h. The measured concentrations confirmed that deviation from the nominal concentrations was less than 20% . No toxic effect and abnormal behavior were observed. Thus, an EC50 (48 h) of > 100 mg/L was derived. 

Description of key information

Daphnia Magna was exposed under static conditions for 48 h to one concentration (100 mg/L) of 3-Morpholinone, 4-[4-[(5S)-5-(aminomethyl)-2-oxo-3-oxazolidinyl]phenyl]-, hydrochloride. No toxic effect was observed.

Additional information

No toxic effects could be determined for 3-Morpholinone, 4-[4-[(5S)-5-(aminomethyl)-2-oxo-3-oxazolidinyl]phenyl]-, hydrochloride in the acute toxicity test for Daphnia.