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EC number: 814-217-0 | CAS number: 353258-35-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid
- Cas Number:
- 353258-35-2
- Molecular formula:
- C9H4ClF3N2O2
- IUPAC Name:
- 8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid
1
- Specific details on test material used for the study:
- Test substance: IN-QEK31-011
Batch No.: SG0312574
Purity: 98.2%
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Remarks:
- The test item was directly dosed into each test flask and pure water was added.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
Study design
- Test type:
- not specified
- Water media type:
- other: pure water
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 ± 2°C during pre-incubation (3 hours) and evaluation period
- pH:
- pH of the activated sludge was 6.7
- Nominal and measured concentrations:
- Nominal: 10, 32, 100, 320 and 1000 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: total and nitrification respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: heterotrophic respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 406.7 mg/L
- 95% CI:
- >= 295 - <= 550.8
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 550.1 mg/L
- 95% CI:
- >= 459.5 - <= 663.7
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: heterotrophic respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 211.9 mg/L
- 95% CI:
- >= 90.8 - <= 493.5
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: nitrification respiration
- Results with reference substance (positive control):
- The 3-hour EC50 for total respiration was found to be 4.3 mg/L (lower cl: 2.3 mg/L, higher cl: 7.8 mg/L) thus lying in the range of 2 - 25 mg/L recommended by the test guidelines. The 3-hour EC50 for heterotrophic respiration was found to be 11.5 mg/L (lower cl: 8.1 mg/L, higher cl: 16.5 mg/L) thus lying in the range of 5 - 40 mg/L recommended by the test guidelines. For the oxygen uptake due to nitrification the 3-hour EC50 was 1.0 mg/L (lower cl: 0.3 mg/L, higher cl: 3.7 mg/L) lying in the range of 0.1 - 10 mg/L recommended by the test guidelines. The result confirms the suitability of the activated sludge used.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC could be established to be 100 mg/L for total and nitrification respiration and 320 mg/L for heterotrophic respiration. The 3-hour EC50 was found to be 406.7 mg/L (lower cl: 295.0 mg/L, higher cl: 550.8 mg/L, total respiration), 550.1 mg/L (lower cl: 459.5 mg/L, higher cl: 663.7 mg/L, heterotrophic respiration) and 211.9 mg/L (lower cl: 90.8 mg/L, higher cl: 493.5 mg/L, nitrification respiration).
- Executive summary:
The effects of the test item on activated sludge were determined in a 3-hour exposure laboratory study conducted under OECD Guideline 209 (2010). Activated sludge from the aeration tank of a municipal sewage treatment plant was exposed to the test item at concentrations of 10, 32, 100, 320 and 1000 mg/L and 6 untreated controls. The same test design was repeated with the nitrification inhibitor N-allylthiourea.
The respiration rate (oxygen consumption) (total respiration, heterotrophic respiration and nitrification) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various concentrations of the test item after an incubation period of 3 hours. The test item was directly dosed into each test flask, which was subsequently filled with pure water. The test item was dissolved into the pure water as homogeneously as possible.
The test item had no inhibiting toxic effects on activated sludge up to a concentration of 100 mg/L, a test item concentration of 1000 mg/L caused an almost complete inhibition of respiration of activated sludge bacteria. The NOEC was established to be 100 mg/L for total respiration and respiration by nitrifying bacteria. The inhibition was 31% and 98% at test item concentrations of 320 mg/L and 1000 mg/L for total respiration. The heterotrophic respiration of the activated sludge was not inhibited up to concentrations of 320 mg/L and almost complete inhibited at 1000 mg/L.
The NOEC could be established to be 100 mg/L for total and nitrification respiration and 320 mg/L for heterotrophic respiration. The 3-hour EC50 was found to be 406.7 mg/L (lower cl: 295.0 mg/L, higher cl: 550.8 mg/L, total respiration), 550.1 mg/L (lower cl: 459.5 mg/L, higher cl: 663.7 mg/L, heterotrophic respiration) and 211.9 mg/L (lower cl: 90.8 mg/L, higher cl: 493.5 mg/L, nitrification respiration).
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