Registration Dossier
Registration Dossier
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EC number: 236-437-0 | CAS number: 13368-45-1
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The toxicity of the test item was determined according to OECD 202 (2004). The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L nominal concentration.
For each test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilized daphnia were counted. No concentration showed toxicity. None of the animals were immobilized in the blank control. As the test item is hydrolyzed in aqueous medium fast, the hydrolysis product Dimethylamine (DMA) was analyzed in this study in accordance to OECD guidance series on testing and assessments No. 23.. At the start and at the end of the test, the content of DMA in the test solutions was determined using a LC/MS/MS- method. The concentrations determined at the start of the test were between 110 % and 122 % of
the nominal concentration. At the end of the test, the measured concentrations were between 116 % and 126 % of the nominal concentration. As no toxicity was observed only the
highest concentration was used for evaluation. The measured concentration in the highest test concentration of 100 mg/L was 114 % and 116 % of the nominal concentrations. Therefore, the determination of the biological results was based on the nominal concentration. 3,5-Dichlorphenol (C6H4Cl2O, CAS No. 591-35-5) was used as positive controls in a current reference study (202204R201) to assure that the test conditions are reliable. The 48 h-EC50-value against 3,5-Dichlorphenol was determined as 2.04 mg/L. The following results were determined for the test item: 48h-EC50> 100 mg/L (nominal)
All validity criteria were met and the result of the test is considered valid.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
As the test item is hydrolyzed in aqueous medium fast, the hydrolysis product Dimethylamine (DMA) was analyzed in this study in accordance to OECD guidance series on testing and assessments No. 23.
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