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EC number: 236-437-0 | CAS number: 13368-45-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
A CO2Headspace Test according to OECD 310 (September 2014) was conducted in order to investigate the ready biodegradability of the test item by measuring the net increase of total inorganic carbon (TIC) in sealed serum flasks with a headspace volume of about a third of the total flask volume. The test item, as sole source of carbon corresponding to 10 mg TOC/L (standard concentration), respectively 2 - 40 mg TOC/L (accepted concentration range), is added to a mineral salts medium, and is inoculated with activated sludge (4 mg d.s./L). The test flasks are sealed and incubated at 20 - 25°C in the dark for 28 days while being shaken. Degradation is followed by determining the carbon dioxide produced via TIC-measurement (TIC = total inorganic carbon) after absorption by sodium hydroxide.On day 6, 13, 20 and 28 samples for TIC-measurements were taken. On each sampling date three flasks of each test series were withdrawn and 1 mL NaOH (7 M) was added through the septa for CO2-absorption in the liquid phase. TIC measurement was performed with a total carbon analyser (TOC-L Shimadzu with an autosampler) by purging the inorganic carbon with H3PO4(25%) using a non-dispersive infrared (NDIR) detector.
To check the activity of the test system, sodium benzoate was used as functional control. This reference compound reached the pass level for ready biodegradability within 6 days (77.8%). The degradation extent in the toxicity control was 50.4% within 28 days indicating no inhibitory effect on the inoculum.
There was no degradation of the test item observed within the test duration of 28 days, the mean degradation extent at the end of the test is 0.01%. The degradation extent was for the most part slightly negative. At the end of the test the pH in the remaining vessels was measured as 8.9. A slight inhibiting effect of the pH on the inoculum cannot be excluded and could be an explanation for the very low degradation extents of the test item in the test vessels. In conclusion the test item did not reach the criteria for ready biodegradability and the test is valid according to OECD test guideline 310 (26 September 2014).
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
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