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EC number: 419-560-6 | CAS number: 4369-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 March - 23 March 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Previous version (1984)
- Deviations:
- yes
- Remarks:
- Hardness (109 mg/l) was lower than the recommended range (140-250 mg/l). Stability tests indicated the parent substance is not stable, however static conditions were used.
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and 100 mg/l
- Sampling method: replicates 1 and 2 were pooled, and 3 and 4
- Sample storage conditions before analysis: - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test material was dispersed in dechlorinated tap water with the aid of shaking and ultrasonic disruption. Volume was adjusted to 2 litres to give the 100 mg/l test concentration. pH adjusted to 7.5.
- Eluate: Not reported
- Differential loading: No
- Controls: dechlorinated tap water
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): In preliminary tests, a 100 mg/l test concentration solution was prepared by direct mixing of the undiluted test material and mixed thoroughly in a glass mixing vessel by magnetic stirrer.
- Other relevant information: - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Not reported
- Method of breeding: Culture conditions ensured reproduction by parthenogenesis. Gravid adults were isolated 24 hours prior to the initiation of the test
- Source: Institut National de Recherche Chimique Appliquee
- Age of parental stock (mean and range, SD): Not reported
- Feeding during test : daphnids were not fed during the test.
ACCLIMATION : not reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 109 mg/l as CaCO3
- Test temperature:
- 21 degrees C (+/- 0 degrees)
- pH:
- 7.5 - 7.6
- Dissolved oxygen:
- 7.9 - 8.4 mg O2/l
- Salinity:
- Not reported
- Conductivity:
- Not reported
- Nominal and measured concentrations:
- Nominal: control and 100 mg/l
Measured: control and 84 (pooled replicates 1 and 2) and 97 (pooled replicates 3 and 4) mg/l at 0 h. Analysis of test solutions at 48 hours showed a marked decline in measured test concentrations and no parent substance was detected. See results section for further details. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml glass stoppered conical flasks
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, 250 ml, fill volume was 250 ml
- Volume of solution: 250ml
- Aeration: not reported
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Total organic carbon: Typical TOC from tests conducted on tap water from 1/1/1994 - 31/12/1994: 1.8 mg/l
- Particulate matter: not reported
- Metals: Many metals analysed from tests conducted on tap water from 1/1/1994 - 31/12/1994, none were found in excessive concentrations.
- Pesticides: Typical total pesticides from tests conducted on tap water from 1/1/1994 - 31/12/1994: <0.03 µg/l
- Chlorine: Typical water total chlorine from tests conducted on tap water from 1/1/1994 - 31/12/1994: 0.23 mg/l
- Alkalinity: Typical water alkalinity from tests conducted on tap water from 1/1/1994 - 31/12/1994: 104 mg/l
- Ca/mg ratio: not reported
- Conductivity: Typical water conductivity from tests conducted on tap water from 1/1/1994 - 31/12/1994: 374 M/CSM
- Salinity: not reported
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0, 24 and 48 hours
OTHER TEST CONDITIONS
- Adjustment of pH: adjusted to pH 7.5
- Photoperiod: 16 hours light: 8 hours dark
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility, other adverse reactions were recorded at 24 and 48 hours.
VEHICLE CONTROL PERFORMED: no - no vehicle used
RANGE-FINDING STUDY
- Test concentrations: 0.10, 1.0, 10, 100 mg/l in static conditions. 10 and 100 mg/l in flow through conditions
- Results used to determine the conditions for the definitive study: No immobilisation found in any concentration. Test material formed a large globule under the flow through conditions so the static conditions were selected for the definitive study. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
None reported
- Observations on body length and weight: not measured
- Other biological observations: None reported
- Mortality of control: No mortality in any treatment
- Other adverse effects control: Not reported
- Immobilisation of control: No immobilisation in any treatment
- Abnormal responses: None reported
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None reported
- Effect concentrations exceeding solubility of substance in test medium: A large globule of test material formed in the range finding study in flow through conditions. However, no evidence of the test concentrations exceeding the solubility of the test substance in the definitive, static study was found. - Reported statistics and error estimates:
- EC50 values were given by inspection of the immobilisation data
- Validity criteria fulfilled:
- yes
- Remarks:
- < 10% immobilisation was observed in the control (0%) and dissolved oxygen was >3 mg/l throughout the test (7.9 - 8.4 mg O2/l)
- Conclusions:
- A 48-hour EC50 value of >100 mg/l was reported for the effects of the 3-(trimethoxysilyl)propyl acrylate on the mobility of Daphnia magna in a limit test, based on nominal concentrations.
Reference
Table 1. Cumulative immobilisation in the static, range-finding study
Nominal concentration (mg/l) |
Cumulative immobilised Daphnia (out of 10 organisms per replicate) |
|
24 hours |
48 hours |
|
Control |
0 |
0 |
0.10 |
0 |
0 |
1.0 |
0 |
0 |
10 |
0 |
0 |
100 |
0 |
0 |
Table 2. Cumulative immobilisation in the flow-through, range-finding study
Nominal concentration (mg/l) |
Cumulative immobilised Daphnia (out of 10 organisms per replicate) |
|
24 hours |
48 hours |
|
Control |
0 |
0 |
10 |
0 |
0 |
100 |
0 |
0 |
Table 3. Cumulative immobilisation in the definitive study
Nominal concentration (mg/l) |
Replicate number |
Cumulative immobilised Daphnia (out of 10 organisms per replicate) |
|
24 hours |
48 hours |
||
Control |
1 |
0 |
0 |
2 |
0 |
0 |
|
100 |
1 |
0 |
0 |
2 |
0 |
0 |
|
3 |
0 |
0 |
|
4 |
0 |
0 |
Table 4. Verification of the test concentrations in the definitive study
Samples |
Nominal concentration (mg/l) |
Measured concentration (mg/l) |
% of nominal concentration |
0 hours |
100 replicates 1+2 |
96.6 |
97 |
|
100 replicates 3+4 |
84.1 |
84 |
48 hours |
100 replicates 1+2 |
<LOD |
- |
|
100 replicates 3+4 |
<LOD |
- |
Description of key information
48-hour: EC50 >100 mg/l based on the mobility of Daphnia magna, based on nominal concentrations. The EC50 is equivalent to >82 mg/l when expressed in terms of concentration of the silanol hydrolysis product, 3-acryloxypropylsilanetriol.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 82 mg/L
Additional information
A 48-hour EC50 value of >100 mg/l based on the mobility of Daphnia magna was determined in a reliable, limit test conducted under static conditions with the registration substance, 3-(trimethoxysilyl)propyl acrylate (CAS 4369-14-6, EC 419-560-6), in accordance with OECD Test Guideline 202. A 24-hour EC50 value and 48-hour NOEC value of >100 mg/l and =100 mg/l, respectively, were also determined in the same study. Gas chromatography analysis determined concentrations of the parent test substance were within 80-120% of nominal values at 0 h, however no parent was detected in test solutions after 48 hours. The results are expressed in terms of the nominal concentrations of the test substance.
The test substance is subject to rapid hydrolysis and under the test conditions it is therefore likely that exposure will have been to its hydrolysis products (3-acryloxypropylsilanetriol and methanol).
The results may be expressed in terms of concentration of the silanol hydrolysis product, 3-acryloxypropylsilanetriol, by applying a molecular weight correction: (MW of silanol = 192.25 / MW of parent = 234.33) * [Concentration of parent = >100 mg/l] = >82 mg/l
There is no basis to expect that methanol significantly influences the results of the test. The toxicity of methanol is discussed further in Section 6.1 Aquatic Toxicity endpoint summary of IUCLID.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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