Reaction mass of glycerol, glycerol 1-formate, glycerol 2-formate, glycerol 1,2-diformate, glycerol 1,3-diformate and glycerol triformate

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)

Version / remarks:

2006

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Reported as: Acidgen FG3

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Supplied by a submersible pump situated on Sutherland’s pier on the west side of Flotta in Scapa Flow, Orkney, Scotland. The site can be referenced from Latitude 58°50'8.02"N, Longitude 3° 8'10.80"W.

Test organisms (species):

Skeletonema costatum

Details on test organisms:

Pre-cultures in the exponential growth phase were prepared from stock laboratory cultures by inoculating treated seawater with nutrient medium (culture medium) to a cell density of approximately 2 x 10E3 to 10E4 cells per millilitre. The pre-cultures were incubated at approximately 20±2 °C under constant illumination for 3d ±1d, and were used as the inoculum source for subsequent toxicity tests.

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

19.2-20.7 °C

pH:

7.37-8.00

Salinity:

34.4-34.5 ppt

Nominal and measured concentrations:

10, 32, 100, 320, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml borosilicate glass conical flasks, to which 50 ml of test medium seawater was added
- Type (delete if not applicable): loosely covered with aluminium foil caps
- initial cell density of approximately 10,000 cells per ml
- No. of vessels per concentration: five concentrations and three replicates per concentration and six controls

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol conducted within the previous three months

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

74.31 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

The test was conducted in accordance with the study plan except for the following deviations but met all other relevant validity criteria.

The pH at 0 hour was out with the guideline criteria. Several attempts were made to adjust the pH to within the validity criteria however due to the nature of the test material this was deemed too difficult.

The coefficient of variation is out with the guideline criteria by 1.29%. The control data met all of the other requirements so this is thought to have had no impact on the result.

Validity criteria fulfilled:

no

Remarks:

The coefficient of variation is out with the guideline criteria by 1.29%. The control data met all of the other requirements so this is thought to have had no impact on the result.

Conclusions:

Acidgen FG3 exhibited a 72h ErC50 value of 74.31mg/L (dilution series) to the marine phytoplankton Skeletonema costatum in the aqueous phase.

Executive summary:

The toxicity of the test substance was assessed in marine water with the marine algae Skeletonema costatum according to ISO 10253 2006 Water quality – Marine algal growth inhibition test with Skeletonema costatum.

 

The definitive tests employ five concentrations and three replicates per concentration (10, 32, 100, 320, or 1000 mg/L) and six controls. The test was conducted in accordance with the study plan except for the following deviations but met all other relevant validity criteria. The pH at 0 hour was out with the guideline criteria. Several attempts were made to adjust the pH to within the validity criteria however due to the nature of the test material this was deemed too difficult. The coefficient of variation is out with the guideline criteria by 1.29%. The control data met all of the other requirements so this is thought to have had no impact on the result.

The test substance exhibited a 72h ErC50 value of 74.31mg/L (dilution series) to the marine phytoplankton Skeletonema costatum in the aqueous phase. The results are from repeat testing due to growth at the top concentration in the first definitive and poor growth at the lowest concentration in the second definitive.

Description of key information

The toxicity of the test substance was assessed in marine water with the marine algae Skeletonema costatum according to ISO 10253 2006 Water quality – Marine algal growth inhibition test with Skeletonema costatum.

 

The definitive tests employ five concentrations and three replicates per concentration (10, 32, 100, 320, or 1000 mg/L) and six controls. The test was conducted in accordance with the study plan except for the following deviations but met all other relevant validity criteria. The pH at 0 hour was out with the guideline criteria. Several attempts were made to adjust the pH to within the validity criteria however due to the nature of the test material this was deemed too difficult. The coefficient of variation is out with the guideline criteria by 1.29%. The control data met all of the other requirements so this is thought to have had no impact on the result.

The test substance exhibited a 72h ErC50 value of 74.31mg/L and the NOEC was 32 mg/L to the marine phytoplankton Skeletonema costatum in the aqueous phase. The results are from repeat testing due to growth at the top concentration in the first definitive and poor growth at the lowest concentration in the second definitive.

Key value for chemical safety assessment

EC50 for marine water algae:

74.31 mg/L

EC10 or NOEC for marine water algae:

32 mg/L