Reaction mass of glycerol, glycerol 1-formate, glycerol 2-formate, glycerol 1,2-diformate, glycerol 1,3-diformate and glycerol triformate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: OSPAR Commission 2006 Part B. Protocol for a fish acute toxicity test

Version / remarks:

consistent with OECD 203 guideline as adapted by OSPAR

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

consistent with OECD 203 guideline as adapted by OSPAR Commission 2006 Part B for marine testing of offshore chemicals

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Reported as: Acidgen FG3

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Natural local seawater is pumped to holding tanks from Scapa Flow, Orkney. The treatment process was: sand filtered to remove particulate, filtered to 1 µm, heated to 16 ºC, UV treated, degassed and held at a quantity of 3000 to 5000 litres. The water is constantly pumped through the system and trickle replenished as the water is used.

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

TEST ORGANISM
- Common name: Sheepshead minnow
- Source: reared and maintained at Opus Maxim Ltd.
- Length at study initiation; 1.5-2.5 cm

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

20±2 ºC

Dissolved oxygen:

97.1-101.8%

Salinity:

33.0-33.3 ‰

Nominal and measured concentrations:

Limit test: 74.31 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 5 litre capacity glass beakers containing 3 litres of test media
- Aeration: Gentle aeration was supplied to all tanks
- Renewal rate of test solution: with replacement of media after 48h
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 74.31 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was reported

Validity criteria fulfilled:

yes

Conclusions:

Acidgen FG3 exhibited no effect at 74.31 mg/L after 96h (directly via seawater) to the marine fish Cyprinodon variegatus in the water phase.

Executive summary:

A 96h limit test in marine water for the test material using Cyprinodon variegatus was conducted according to OSPAR Commission 2006 Part B Protocol for a fish acute toxicity test which is adapted from the OCED TG 203 for the marine environment. The test concentration is based on the LC/EC50 of the test material between most the sensitive species Skeletonema costatum and Acartia tonsa. Testing has indicated that the algal species was the more sensitive from allied studies.

 

Ten fish were exposed to 74.3 mg/L for 96 hours with replacement of media after 48h. No mortality was reported in either the test concentration or the control vessels. All validity criterion were met and the test substance exhibited no effect at 74.31mg/L after 96h (directly via seawater) to the marine fish Cyprinodon variegatus in the water phase. 

Description of key information

A 96h limit test in marine water for the test material using Cyprinodon variegatus was conducted according to OSPAR Commission 2006 Part B Protocol for a fish acute toxicity test which is adapted from the OCED TG 203 for the marine environment. The test concentration is based on the LC/EC50 of the test material between most the sensitive species Skeletonema costatum and Acartia tonsa. Testing has indicated that the algal species was the more sensitive from allied studies.

 

Ten fish were exposed to 74.3 mg/L for 96 hours with replacement of media after 48h. No mortality was reported in either the test concentration or the control vessels. All validity criterion were met and the test substance exhibited no effect at 74.31mg/L after 96h (directly via seawater) to the marine fish Cyprinodon variegatus in the water phase. 

Key value for chemical safety assessment

Marine water fish

Marine water fish

Effect concentration:

74.3 mg/L