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EC number: 272-823-5 | CAS number: 68916-18-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 04 to 30 November 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: CF02
- Expiration date of the lot/batch: 31.01.2019
- Purity test date: not available
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at 4°C, in the dark
- Stability under test conditions: not available
- Solubility and stability of the test substance in the solvent/vehicle: water solubility 2.85 mg/L
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not applicable
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Due to the low limit of water solubility of the test item Water accommodated Fraction (WAF) were tested. Supersaturated stock suspensions of the test item were prepared separately by suspending test item in test water at concentrations of 100, 50, 25, 12.5 and 6.25 mg test item/L.
- Preliminary purification step (if any): none
- Final dilution of a dissolved solid, stock liquid or gel: not applicable
- Final preparation of a solid: not applicable
OTHER SPECIFICS: none - Analytical monitoring:
- yes
- Details on sampling:
- Test solutions were sampled from below the water surface via a drain that was approximately 5 cm above the vessel bottom.
- Vehicle:
- yes
- Remarks:
- Reconstituted water
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout (Oncorhynchus mykiss)
- Strain: not specified
- Source: Forellenzuchtbetrieb Störk, 88348 Bad Saulgau, Germany
- Age at study initiation (mean and range, SD): not specified
- Length at study initiation (length definition, mean, range and SD): 4.24 ± 0.3 cm
- Weight at study initiation (mean and range, SD): 0.81 ± 0.1 g
- Method of breeding: not specified
- Maintenance of the brood fish: not specified
ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions (same as test or not): held in water of the quality to be used in the test for at least seven days immediately before testing
- Type and amount of food during acclimation: not specified
- Feeding frequency during acclimation: three times per week or daily until 24 hours before the test was started
- Health during acclimation (any mortality observed): no mortality observed
FEEDING DURING TEST: no - Test type:
- static
- Water media type:
- other: Reconstituted water (ISO medium)
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
- Hardness:
- 2.5 mmol/L
- Test temperature:
- 14.-16.4°C
- pH:
- 7.5-7.9
- Nominal and measured concentrations:
- 6.25, 12.5, 25, 50 and 100 mg/L loading rate Water Accommodated Fraction
- Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 12 liter glass aquaria
- Aeration: 98 to 100 % of the air saturation value
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not applicable
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (ISO medium)
- Total organic carbon: 10.721 mg C/L at 0h
- Particulate matter: not specified
- Metals: not specified
- Pesticides: not specified
- Chlorine: not specified
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1 (based on molarity)
- Culture medium different from test medium: no
- Intervals of water quality measurement: not specified
OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: 16h light: 8h dark
- Light intensity: 650 – 730 lux
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: none, a limit test was performed at 100 mg/L WAF prior to dose response test
- Test concentrations: 6.25, 12.5, 25, 50 and 100 mg/L loading rate Water Accommodated Fraction
- Results used to determine the conditions for the definitive study: sub-lethal effects and mortality - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- other: NOEL
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF
- Basis for effect:
- other: sub-lethal effects
- Key result
- Duration:
- 96 h
- Dose descriptor:
- other: LOEL
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF
- Basis for effect:
- other: strong ventilation
- Details on results:
- - Behavioural abnormalities: some fish remained at the water surface, possibly caused by the coloration of the test medium
- Observations on body length and weight: not specified
- Other biological observations: not specified
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: strong ventilation at 100 mg/L loading rate WAF
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not specified
- Effect concentrations exceeding solubility of substance in test medium: none - Reported statistics and error estimates:
- No statistical analysis was performed
- Sublethal observations / clinical signs:
See attached additional information on results.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an acute toxicity test performed on Rainbow trout, and conducted according to OECD test guideline 203 and to GLP, the NOEL and LOEL values were determined at 50 and 100 mg/L loading rate as a Water Accommodated Fraction (WAF) after 96 hours of exposure to Coffee, bean, roasted, ext. The 96-hour LC50 was determined to be > 100 mg/L loading rate WAF.
- Executive summary:
The acute toxicity of Coffee, bean, roasted, ext. to rainbow trout (Oncorhynchus mykiss) was determined in dose response test according to the OECD test guideline 203 and to GLP.
Due to the low aqueous solubility and complex nature of the test item, the test medium was prepared as a Water Accommodated Fraction (WAF).
Seven fish were exposed to a WAF of the test item at nominal loading rates of 0, 6.25, 12.5, 25, 50 and 100 mg/L for 96 hours under static conditions. Morality and sub-lethal effects were recorded after 24, 48, 72 and 96 hours.
No mortality was observed in the control and the test item concentration up to 100 mg/L loading rate WAF. At 100 mg/L loading rate WAF, all fish displayed strong ventilation shortly after insertion into the test concentration.
Some fish were observed mainly at the water surface at 100 mg/L loading rate WAF after 48, 72 and 96 hours. At 72 hours 3 fish were observed mainly at the water surface at 50 mg/L loading rate WAF. The position “mainly at the water surface” was possibly caused by the coloration of the test medium and was not judged as sub-lethal effect and not considered for the NOEL / LOEL determination.
During the test the fish were exposed to 21.1 and 10.3 mg Carbon/L of the nominal loadings of 100 and 50 mg test item/L, respectively. The results for nominal loadings of 25, 12.5 and 6.25 mg Carbon/L were below the Limit of Quantification, however, they were clearly below the NOEL.
The NOEL and LOEL after 96 hours of exposure were determined at 50 and 100 mg/L loading rate WAF respectively.
The 96-hour LC50 was determined to be > 100 mg/L loading rate WAF.
Reference
Description of key information
NOEL: 50 mg/L loading rate WAF
LOEL: 100 mg/L loading rate WAF
LC50: >100 mg/L loading rate WAF
Key value for chemical safety assessment
Additional information
The acute toxicity of Coffee, bean, roasted, ext. to rainbow trout (Oncorhynchus mykiss) was determined in dose response test according to the OECD test guideline 203 and to GLP.
Due to the low aqueous solubility and complex nature of the test item, the test medium was prepared as a Water Accommodated Fraction (WAF).
Seven fish were exposed to WAFs of the test item at nominal loading rates of 0, 6.25, 12.5, 25, 50 and 100 mg/L for 96 hours under static conditions. Morality and sub-lethal effects were recorded after 24, 48, 72 and 96 hours.
No mortality was observed in the control and the test item concentration up to 100 mg/L loading rate WAF. At 100 mg/L loading rate WAF, all fish displayed strong ventilation shortly after insertion into the test concentration.
Some fish were observed mainly at the water surface at 100 mg/L loading rate WAF after 48, 72 and 96 hours. At 72 hours 3 fish were observed mainly at the water surface at 50 mg/L loading rate WAF. The position “mainly at the water surface” was possibly caused by the coloration of the test medium and was not judged as sub-lethal effect and not considered for the NOEL / LOEL determination.
During the test the fish were exposed to 21.1 and 10.3 mg Carbon/L of the nominal loadings of 100 and 50 mg test item/L, respectively. The results for nominal loadings of 25, 12.5 and 6.25 mg Carbon/L were below the Limit of Quantification, however, they were clearly below the NOEL.
The NOEL and LOEL after 96 hours of exposure were determined at 50 and 100 mg/L loading rate WAF respectively.
The 96-hour LC50 was determined to be > 100 mg/L loading rate WAF.
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