2-cyclohexylethyl acetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-cyclohexylethyl acetate

EC Number:

244-543-3

EC Name:

2-cyclohexylethyl acetate

Cas Number:

21722-83-8

Molecular formula:

C10H18O2

IUPAC Name:

2-cyclohexylethyl acetate

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

The primary stock solution used in the range-finding test was prepared by dissolving 10 mg of the
test substance in 40 mL of dilution water — M4 and the total volume was completed to 100 mL,
resulting in a concentration of 100 mg.L-1. The secondary stock solutions of the 10; 1 and 0,1
mg.L-lwere prepared by dilutions of the primary stock solution.
The primary stock solution used in the definitive test was prepared by dissolving 50 mg of the test
substance in 40 mL of dilution water — M4 and the total volume was completed to 500 mL,
resulting in a concentration of 100 mg.L-1.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The test system used for this study is described below, according to Straus (1820):
Kingdom: Animalia
Phylum: Arthropoda
Subphylum: Crustacea
Class: Branchiopoda
Order: Cladocera
Family: Daphniidae
Genus: Daphnia
Species: Daphnia magna
Young Daphnia magna 5 24 hours old were obtained from the culture kept at the Ecotoxicology
Laboratory, maintenance and cultivation records are archived at BIOAGRI.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

196.7 mg.L-1 CaCO3

Test temperature:

20.1°C

pH:

7.7

Dissolved oxygen:

8.0 mg.L-1

Details on test conditions:

In each culture container 30 organisms were maintained daily. They were daily fed with algae
suspension (Pseudokirchneriella subcapitata) with 107 cells.organisms-1. The water of each
culture was renewed twice a week (Mondays and Thursdays).

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 36.82 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mortality

Details on results:

The 24 and 48-hours EC50 values of the product Cyclohexyl Ethyl Acetate were 50.00 mg.L-1 (95%
confidence limits = 34.76 to 71.92 mg.L-1) and 36.82 mg.L-1 (95% confidence limits = 30.08 to 45.07
mg.L-1), respectively.
In the definitive test the highest tested concentration that did not cause acute effect (immobility)
to the organisms after 48 hours was 13 mg.L-1. The lowest concentration that caused 100%
immobility was 100 mg.L-1.

Results with reference substance (positive control):

The 24-hours EC50 value of the reference substance (potassium dichromate) was 1.05 mg.L-1 (95%
confidence limits = 0.91 to 1.21 mg.L-1), showing the sensitivity of the test system (ISO 6341 /
OECD 202, 2004).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hours EC50 value of Cyclohexyl Ethyl Acetate was 36.82 mg.L-1 (95% confidence limits = 30.08 to 45.07 mg.L-1).