Lauric acid, monoester with propane-1,2-diol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 November 2016 - 14 December 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Lot/batch No.: 1023R25811
- Expiration date of the lot/batch: 14 September 2019
- Purity: 100%
- Storage conditions: Room temperature in the dark

Analytical monitoring:

yes

Remarks:

gas chromatography

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.55 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.6 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

The detection system was found to have acceptable linearity. The analytical procedure was found to have acceptable recoveries of test item in test medium. The method of analysis was validated and proven to be suitable for use.

The limit of quantification (LOQ) was determined by calculating the sample concentration that gave a peak equivalent to ten times the baseline noise. Using this method the LOQ was determined to be 0.067 mg/L.

A sub-lethal effect of exposure was observed in the 0.59 mg/L test concentration.

The No Observed Effect Concentration after 24 and 48 hours exposure was 0.23 mg/L. The Lowest Observed Effect Concentration after 24 and 48 hours exposure was 0.59 mg/L.

The slopes and their standard errors of the response curves at 24 and 48 hours were 5.0 (SE = 0.046) and 14.7 (SE = 0.33) respectively.

The test was considered to be valid given that none of the control daphnids showed immobilization or other signs of disease or stress and that the oxygen concentration at the end of the test was equal to or greater than 3 mg/L in the control and test vessels.

Validity criteria fulfilled:

yes

Conclusions:

Exposure of Daphnia magna to the test item has been investigated and gave the following results based on the geometric mean measured test concentrations:
48 hours:
EC50 = 0.60 mg/L
No Observed Effect Concentration (NOEC) = 0.23 mg/L
Lowest Observed Effect Concentration (LOEC) = 0.59 mg/L

Description of key information

48-Hour Acute Immobilization Test in Daphnia sp.

EC50 = 0.60 mg/L

No Observed Effect Concentration (NOEC) = 0.23 mg/L

Lowest Observed Effect Concentration (LOEC) = 0.59 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.6 mg/L