Registration Dossier
Registration Dossier
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EC number: 226-901-0 | CAS number: 5538-94-3
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The key study (Krize, 1978) is a reliable (Klimisch 2) acute toxicity using the test substance, dioctyldimethyl ammonium chloride (C8DAQ). The test substance was exposed to rainbow trout Oncorhynchus mykiss in a static test, resulting in a 96-hour LC50 of 0.7 mg/l (test substance as received) and a 96-hour LC50 of 0.35 mg a.i./l (corrected for the active ingredient (a.i.) content). The study follows the principles of OECD 203 and was undertaken by a commercial laboratory. This study is selected as the Key Study for its reliability, use of the test substance C8DAQ, and the conservative LC50 compared to the other acute toxicity to fish studies. In addition, the NOEC value reported for the study (NOEC of 0.06 mg ai/l) is lower than NOEC values reported for the two fish species reported in the Springborn Laboratory studies (1994).
A reliable (Klimisch 2) supporting study (Krize and Gilman, 1973) was conducted using the test substance, dioctyldimethyl ammonium chloride (C8DAQ). Three fish species were used; fathead minnow, bluegill sunfish and channel catfish. The three different freshwater fish species were exposed to the test substance in a static test, resulting in 96-hour LC50s of 5.2, 5.9, 11.2 mg/l (test substance as received) for fathead minnow, bluegill sunfish and channel catfish, respectively. When corrected for a.i. content the LC50s were 2.6, 2.95 and 5.6 mg ai/l, respectively. Further supporting studies are available for the read-across substance DDACarbonate (Springborn Laboratory, 1994). LC50 are available for bluegill sunfish (Lepomis macrochirus) of 0.28 mg ai/l, the sheepshead minnow (Cyprinodon variegatus) of 1.1 mg ai/l and the rainbow trout (Oncorhynchus mykiss) of 0.810 mg ai/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.35 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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