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EC number: 224-030-0 | CAS number: 4170-30-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: full report available + GLP
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Guideline:
- other: see below
- Principles of method if other than guideline:
- Klecak et al. Joran, 1982 (contact dermatitis 8:109-116, 1982.)
- GLP compliance:
- yes
- Type of study:
- open epicutaneous test
Test material
- Reference substance name:
- Crotonaldehyde
- EC Number:
- 224-030-0
- EC Name:
- Crotonaldehyde
- Cas Number:
- 4170-30-3
- Molecular formula:
- C4H6O
- IUPAC Name:
- but-2-enal
- Details on test material:
- Received: 1 April 1988
liquid
stored in the freezer at -20C
incompatible with ketones
Sensitive to exposure to air and heat and may also be sensitive to light
readily soluble DMSO, 95% ethanol and acetone.
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- female
- Details on test animals and environmental conditions:
- Mice obtained from Taconic Farms. The mice were hepatitis and Sendai virus free. Animals arrived at 4-5 weeks of age (17-21 grams).
1 week quarantine
not used on study until 8 weeks of age
housed 4 animals per cage in plastic shoebox cages with sawdust bedding
Zielger Rat and Mouse Ration (NIH 031)
tap water ad libitum
temperature " 21-24C
relative humidity: 40-60%
12 hour light/dark cycle
cages were cleaned and sanitized twice per week
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- epicutaneous, open
- Vehicle:
- other: 4 parts acetone one part olive oil
- Concentration / amount:
- 10% crotonaldehyde with challenge at 0.3, 1 and 3% crotonaldehyde
Challengeopen allclose all
- Route:
- epicutaneous, open
- Vehicle:
- other: 4 parts acetone one part olive oil
- Concentration / amount:
- 10% crotonaldehyde with challenge at 0.3, 1 and 3% crotonaldehyde
- No. of animals per dose:
- 8 mice / group
- Positive control substance(s):
- yes
- Remarks:
- 1-fluoro-2,4-dinitrobenzene (DNFB)
Results and discussion
- Positive control results:
- hypersensitivity index = 7.5
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 0
- No. with + reactions:
- 0
- Total no. in group:
- 8
- Clinical observations:
- no data
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 48.0. Group: negative control. Dose level: 0. No with. + reactions: 0.0. Total no. in groups: 8.0. Clinical observations: no data.
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 0.3%
- No. with + reactions:
- 0
- Total no. in group:
- 8
- Clinical observations:
- no data
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 48.0. Group: test group. Dose level: 0.3%. No with. + reactions: 0.0. Total no. in groups: 8.0. Clinical observations: no data.
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 1.0%
- No. with + reactions:
- 0
- Total no. in group:
- 8
- Clinical observations:
- no data
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 48.0. Group: test group. Dose level: 1.0%. No with. + reactions: 0.0. Total no. in groups: 8.0. Clinical observations: no data.
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 3%
- No. with + reactions:
- 0
- Total no. in group:
- 8
- Clinical observations:
- no data
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 48.0. Group: test group. Dose level: 3%. No with. + reactions: 0.0. Total no. in groups: 8.0. Clinical observations: no data.
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- positive control
- Dose level:
- no data
- No. with + reactions:
- 8
- Total no. in group:
- 8
- Clinical observations:
- no data
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 48.0. Group: positive control. Dose level: no data. No with. + reactions: 8.0. Total no. in groups: 8.0. Clinical observations: no data.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: NA
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: NA
Any other information on results incl. tables
no data
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- negative for skin sensitization potential
- Executive summary:
Crotonaldehyde (CRA) was selected for evaluation as a sensitizing agent for contact sensitivity in guinea pigs and mice. The objective of this study was to determine the sensitizing potential of crotonaldehyde when applied dermally to female B6C3F1 mice.
Crotonaldhyde was tested on female B6C3F1 mice. The doses of crotonaldehyde ranged from 0.3% to 3.0% in a solution of 4 parts acetne to one part olive oli (4:1) for sensitization and challenge. Mice received 20 ul by direct dermal application for 5 consecutive days to a prepared site. DNFB (2,4 -dinitrofluorobenzene) (99.6%) was used as a positive control at a concentration of 0.5%. Measurement of the contact sensitivity was accomplihsed byt he radioisotopic assay.
A dose-dependent contact hypersensitivity response to crotonaldehyde could not be demonstrated in mice.
The study was conducted at the Medical College of Virginia Immunotoxicology Laboratory under NTP Contract No. ES 55094.
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