Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 218-267-9 | CAS number: 2100-42-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-05-04 to 1994-05-19
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well performed GLP-study following a former OECD guideline 471 version.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- former version of OECD 471, without 5th strain (E. coli WP2 uvrA, or E. coli WP2 uvrA (pKM101), or S. typhimurium TA102)
- Deviations:
- no
- Remarks:
- deviations related to the former version
- Principles of method if other than guideline:
- Testing was done without 5th strain (E. coli WP2 uvrA, or E. coli WP2 uvrA (pKM101), or S. typhimurium TA102)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1-chloro-2,5-dimethoxybenzene
- EC Number:
- 218-267-9
- EC Name:
- 1-chloro-2,5-dimethoxybenzene
- Cas Number:
- 2100-42-7
- Molecular formula:
- C8H9ClO2
- IUPAC Name:
- 2-chloro-1,4-dimethoxybenzene
- Details on test material:
- - Name of test material (as cited in study report): Chlorhydrochinondimethylether
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix, rat, treatment: Aroclor1254
- Test concentrations with justification for top dose:
- 0, 4, 20, 100, 500, 2500, 5000 µg per plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Sodium-azide, 9-Aminoacridine, 2-Nitrofluorene, 2-Aminoanthracene
- Details on test system and experimental conditions:
- BACTERIA
Bacteria were grown overnight in nutrient broth (25 g Oxoid Nutrient Broth No. 2 /liter) at approx. 37 °C. The suitable amount of bacteria in the cell suspension was checked by nephelometry. For inoculation, stock cultures which are stored at approx. -80°C, were used. The compound was tested with the strains Salmonella typhimurium TA 98, TA 100, TA 1535 and TA 1537.
TOXICITY EXPERIMENTS AND DOSE RANGE FINDING
The first experiment was performed with all tester strains using three plates per dose to get information on mutagenicity and toxicity for calculation of an appropriate dose range. A reduced rate of spontaneously occuring colonies as well as visible thinning of the bacterial lawn were used as indicator for toxicity. Thinning of the bacterial lawn was evaluated microscopically.
In combination with the second experiment, toxicity testing was performed as follows:
0.1 ml of the different dilutions of the test compound were thoroughly mixed with 0.1 ml of 10^6 dilution of the overnight culture of TA 100 and plated with histidine and biotin rich top agar (3 plates per dose). The solvent control is compared with the number of colonies per plate in the presence of the test compound. Results are given as a ratio of these values (= surviving fraction).
MUTAGENICITY TEST
Top agar was prepared for the Salmonella strains by mixing 100 ml agar (0.6 % (wlv) agar, 0.5 % (w/v) NaCI) with 10 ml of a 0.5 mM histidine-biotin solution.
The following ingredients were added (in order) to 2 ml of molten top agar at approx. 45 °C:
0.1 ml of an overnight nutrient broth culture of the bacterial tester strain
0.1 ml test compound solution
0.5 ml S-9 Mix (if required) or buffer
After mixing, the liquid was poured into a petridish with minimal agar (1.5 % (w/v) agar, Vogel-Bonner E medium with 2 % (w/v) glucose). After incubation for approximately 48 hours at approx. 37 °C in the dark, colonies (his' revertants) were counted.
Two independent experiments were performed. - Evaluation criteria:
- A test article is classified mutagenic if either of the following conditions under a) and b) is achieved:
a) a test article produces at least a 2-fold increase in the mean number of revertants per plate of at least one of the tester strains over the mean number of the appropriate vehicle control at complete bacterial background lawn
b) a test article induces a dose-related increase in the mean number of revertants per plate of at least one of the tester strains over the mean number of revertants per plate of the appropriate vehicle control in at least two to three concentrations of the test article at complete bacterial background lawn.
The test results must be reproducible.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- toxic to most strains at 2500µg/plate and above
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
STERILITY
CHECKS AND CONTROL PLATES
Sterility
of S-9 Mix and the test compound were indicated by the absence of
contamination on the testmaterial
and S-9 Mix sterility check plates. Control plates (background control
and positive controls) gavethe
expected number of colonies.
SOLUBILITY
The
test compound did not precipitate on the plates up to the highest
investigated dose of 5000
microgram/plate.
TOXICITY
The
test compound was tested at doses of 4 to 5000 microgram/plate and
proved to be toxic to most of thebacterial
strains at a dose of 2500 microgram/plate and above.
Thinning
of the bacterial lawn and a reduction in the number of colonies have
been observed at this dose.
Therefore 5000 microgram/plate was chosen as the highest dose in the
second experiment.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Summarizing, it can be stated thatChlorhydrochinondimethylether is not mutagenic in these bacterialtest systems neither with nor without exogenous metabolic activation at the dose levels investigated. - Executive summary:
Chlorhydrochinondimethylether was tested for mutagenicity with the strains TA 100, TA 1535, TA 1537and TA 98 of Salmonella typhimurium.
The mutagenicity studies were conducted in the absence and in the presence of a metabolizing systemderived from rat liver homogenate. The test substance was dissolved in DMSO and a dose range of6 different doses from 4 microgram/plate to 5000 microgram/plate was used.
Control plates without mutagen showed that the number of spontaneous revertant colonies was similiar to that described in the literature. All the positive control compounds gave the expected increase in the number of revertant colonies.
Toxicity: The test compound proved to be toxic to most of the bacterial strains at a dose of 2500 microgram/plate and above.
5000 microgram/plate was chosen as top dose level for the mutagenicity study.
Mutagenicity: In the absence of the metabolic activation system the test compound did not show a dosedependent increase in the number of revertants in any of the bacterial strains. Also in the presence of ametabolic activation system, treatment of the cells with
Chlorhydrochinondimethylether did not result inrelevant increases in the number of revertant colonies.
Summarizing, it can be stated thatChlorhydrochinondimethylether is not mutagenic in these bacterialtest systems neither with nor without exogenous metabolic activation at the dose levels investigated.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.