Butane-1,2-diol

Endpoint summary

Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information

Butane-1,2-diol was not mutagenic to Salmonella typhimurium strains S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 at doses up to 5,000 µg/plate with or without metabolic activation. The test substance also induced neither chromosomal aberrations nor polyploidy in Chinese hamster CHL cells at doses up to 0.9 mg/mL either with or without metabolic activation

Link to relevant study records

Reference

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Qualifier:

according to guideline

Guideline:

JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals

GLP compliance:

yes

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Test concentrations with justification for top dose:

Dose / Concentration: 312.5-5000 µg/ plate.

Vehicle / solvent:

DMSO

Details on test system and experimental conditions:

Exposure comments:
Doses of: 0, 312.5, 625, 1250, 2500 or 5000 micrograms of 1,2-butanediol were applied per plate in 3 plates.
Positive control:
A) S9 negative received AF-2 sodium azide or 9-aminoacridine
B) S9 positive received 2-aminoanthracene. S9 activated by phenobarbital and 5,6-benzoflavone.

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Minimum concentration of test substance at which toxicity to bacteria was observed is > 5000ug/plate with metabolic activation and 5000ug/plate without metabolic activation.

In all strains at all concentrations tested, with or without metabolic activation, the test substance did not induce any changes within the chromosomal chromatin.

Executive summary:

CAS 584-03-2 was considered as "negative" in gene mutation tests under the experimental conditions used.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (negative)