Registration Dossier
Registration Dossier
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EC number: 252-346-9 | CAS number: 35074-77-2
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
An IC50 of above 100 mg/L was determined. (BASF, 1988)
Key value for chemical safety assessment
Additional information
The inhibitory effects of the test substance on the respiration rate of aerobic waste water bacteria was examined in a static test according to OECD guideline 209 (BASF, 1998). Bacteria were cultivated under specific conditions and respiration rate was determined under the influence of test substance compared to blank and positive controls. Therefore the oxygen consumption of the bacteria suspension was measured after the incubation period with the test substance. The test substance was directly measured into the bacteria suspension. As positive control substance 3,5-Dichlorophenol was used in concentrations of 3.2, 10 and 32 mg/L. An IC50 of 14 mg/L was determined for the positive control.
The bacteria were incubated in duplicate with the test substance at the dose level of 100 mg/L (nominal concentration) for 3 hours. The following inhibitory concentrations were determined:
IC20 (3h) > 100 mg/L
IC50 (3h) > 100 mg/L
IC80 (3h) > 100 mg/L
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