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EC number: 227-824-5 | CAS number: 5994-61-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005.03.07-2005.03.11
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Parcom Protocols on Methods fir the Testing of Chemicals used in the Offshore Industry
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-(carboxymethyl)-N-(phosphonomethyl)glycine
- EC Number:
- 227-824-5
- EC Name:
- N-(carboxymethyl)-N-(phosphonomethyl)glycine
- Cas Number:
- 5994-61-6
- Molecular formula:
- C5H10NO7P
- IUPAC Name:
- 2-[(carboxymethyl)(phosphonomethyl)amino]acetic acid
- Details on test material:
- - Name of test material (as cited in study report): PMIE or Phosphonomethyliminodiacetic acid
- Substance type: Solid
- Physical state: White powder
- Analytical purity: 99 % w/w
- Purity test date: 15.06.2005
- Lot/batch No.: SB-7-4-89
- Expiration date of the lot/batch: 11.09.2005
- Stability under test conditions: Stable for 6 months
- Storage condition of test material: at - 20 ˚C
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 200 mg/L analysed (the highest exposure solution concentration)
- Sample storage conditions before analysis: at - 20 ˚C
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Stock solution: Nominiel concentration of the highest exposure solution: 200 mg/L, actual concentration 195 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None
Test organisms
- Test organisms (species):
- Cyprinodon variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: Sheephead minnow
- Source: originally obtained from a stock culture at Opus Plus Ltd.(the former ERT Orkney water Technology Centre, and cultured at DHI since June 2003. Animals from this DHI culture was used for this study
- Length at study initiation (length definition, mean, range and SD):standard length 18.5 ± 2.37 cm
- Weight at study initiation (mean and range, SD): 0.17 ± 0.06 g
- Method of breeding: No data
- Feeding during test: No
ACCLIMATION
- Acclimation conditions : no acclimatation needed, test done under same conditions at same facility as test animals was optained from.
Study design
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 20 ± 0.5 ˚C
- pH:
- 7.9-8.2
- Dissolved oxygen:
- 94 -100 % saturated
- Salinity:
- 32 ‰
- Nominal and measured concentrations:
- Nominial concentrations: 0 (control); 10; 20; 50; 100; 200 mg/L
Measured concentration: 195 mg/L measured for the highest nominial concentration, 200 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquarium
- Type: open
- Material, size, headspace, fill volume: glass, 5 L, ?, 4 L
- Aeration: yes
- No. of organisms per vessel: 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater from the North Sea, filtered through Millipore filters (10; 5; 0.5; 0.22 μm)
- Particulate matter: None
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: light/dark 16:8 hours
TEST CONCENTRATIONS
- Test concentrations: 0 (control);10; 20; 50; 100 and 200 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol, CAS No. : 591-35-5, at 2.3 mg/L.
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- No mortality or any other significant effects were recorded at even the highest concentration, 200 mg/L
- Details on results:
- - Behavioural abnormalities: None
- Observations on body length and weight: None
- Other biological observations: None
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: Not known - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Mortality: 20 % at 2.3 mg/L
- LC50: No calculated
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Mortality in controls under 10 %, oxygen over 60%, mortality with reference test within acceptable range, 20-80 %
- Conclusions:
- LC50(96 h) of N-(carboxymethyl)-N-(phosphonomethyl)-glycine, CAS No. 5994-61-6, exceeds 200 mg/L for Cyprinodon variegatus
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