1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals

Deviations:

no

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione

EC Number:

213-834-7

EC Name:

1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione

Cas Number:

1025-15-6

Molecular formula:

C12H15N3O3

IUPAC Name:

tris(prop-2-en-1-yl)-1,3,5-triazinane-2,4,6-trione

Method

Target gene:

his operon (for S. typhimurium strains)
trp operon (for E. coli strain)

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Metabolic activation system:

cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of rats treated with phenobarbital and 5,6-benzoflavone

Test concentrations with justification for top dose:

without metabolic activation, all strains: 156, 313, 625, 1250, 2500, 5000 µg/plate
with metabolic activation, S. typhimurium strains: 156, 313, 625, 1250, 2500, 5000 µg/plate
with metabolic activation, E. coli strain: 313, 625, 1250, 2500, 5000 µg/plate

Vehicle / solvent:

- Vehicle used: DMSO (0.1 mL per test)
- Justification for choice of vehicle: due to limited solubility of the test substance in water, DMSO was selected as vehicle.

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide (AF2; 0.01 or 0.1 µg/plate, -S9, TA 100, TA 98, E. coli); sodium azide (SA; 0.5 µg/plate, -S9, TA 1535); 9-aminoacridine (9AA; 80 µg/plate, -S9, TA 1537); 2-Aminoanthracene (2AA; 0.5-10 µg/plate, all strains)

Details on test system and experimental conditions:

METHOD OF APPLICATION: Pre-incubation method

DURATION
- Preincubation period: 20 min
- Exposure duration: 48 hours

NUMBER OF REPLICATIONS: 3 replications each in two independent experiments

DETERMINATION OF CYTOTOXICITY
- Method: growth inhibition

Evaluation criteria:

Colonies were counted for 3 plates. Mean values which are at least twice as high as the negative vehicle control are considered as positive. Otherwise it is considered as negative.

Statistics:

Mean values and standard deviation were calculated.

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

at 2500 and 5000 µg/plate with and without S9 mix

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Test results 2

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

at 2500 and 5000 µg/plate without S9 mix

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Additional information on results:

RANGE-FINDING/SCREENING STUDIES: YES; A "cytotoxicity" study was performed with 50, 150, 500, 1500 and 5000 µg/plate with and without S9 mix. Growth inhibition was observed at 5000 µg/plate without S9 mix in all tested strains (TA 98, TA 100, TA 1535, TA 1537 and E. coli WP2 uvrA) and at 5000 µg/plate with S9 mix in all S. typhimurium strains.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The test substance did not induce gene mutations in S. typhimurium strains TA 98, TA 100, TA 1535, TA 1537 and in E. coli WP2 uvrA strain at concentrations up to 5000 µg/plate with and without metabolic activation. Cytotoxicity was observed at and above 2500 µg/plate in all strains without S9 mix and in S. typhimurium strains with S9 mix.