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Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyclododeca-1,5,9-triene
EC Number:
225-533-8
EC Name:
Cyclododeca-1,5,9-triene
Cas Number:
4904-61-4
Molecular formula:
C12H18
IUPAC Name:
cyclododeca-1,5,9-triene
Constituent 2
Reference substance name:
1,5,9-cyclododecatriene
IUPAC Name:
1,5,9-cyclododecatriene
Details on test material:
1,5,9-cyclododecatriene of Hüls AG, purity > 99.5%

Test animals

Species:
rabbit
Strain:
other: Small white Russian, Chbb
Details on test animals or test system and environmental conditions:
TEST ANIMALS: 
- Strain: Small white Russian, Chbb-SPF
- Sex: male and female
- Source: Dr. Karl Thomae GmbH, Biberach (Germany)
- Weight at study initiation: 2.2 - 2.7 kg
- Number of animals: 3 males, 3 females
- Feed: K4 complete feed for rabbits (Ssniff, Soest, Germany);
- Water: tap water ad libitum
Environmental conditions:
- Room temperature: 20°C (+/- 1°C);
- Humidity: 60% (+/- 5%);
- Air change: 15 times/hour;
- Light: 12 hour light/dark rhythm;

Test system

Type of coverage:
occlusive
Preparation of test site:
shaved
Vehicle:
other: no vehicle
Amount / concentration applied:
undiluted
Duration of treatment / exposure:
4 hour(s)
Observation period:
1, 24, 48, 72 hours; 6, 8, 11, 14 days after exposure
Number of animals:
6
Details on study design:
ADMINISTRATION/EXPOSURE 
- Area of exposure: ca. 6 cm2
- Occlusion: mull patch, polyethylene film, elastic dressing 
- Postexposure period: 14 days
- Removal of test substance: dist. water
EXAMINATIONS
- Scoring system: OECD; evaluation: VCI notes on safety data sheet
- Examination time points: 1, 24, 48, 72 hours; 6, 8, 11, 14 days;  evaluation based on readings 1-4

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 1, 24, 48, 72 h
Score:
1.2
Max. score:
8
Irritation parameter:
erythema score
Basis:
mean
Time point:
other: 24, 48, 72 h
Score:
1
Max. score:
4
Irritation parameter:
edema score
Basis:
mean
Time point:
other: 24, 48, 72 h
Score:
0.61
Max. score:
4
Irritant / corrosive response data:
AVERAGE SCORE
- Erythema: 1.00
- Edema: 0.61
Other effects:
no other effects

Any other information on results incl. tables

no further remarks

Applicant's summary and conclusion

Conclusions:
Under the conditions of the study, the test item 1,5,9 -cyclododecatrien showed a slightly irritant effect on the skin of male and female rabbits which leads to no classification according to 67/548/EEC and according CLP regulation (1272/2008).
Executive summary:

To test the acute skin irritant effect the test item 1,5,9 -cyclododecatrien was applied to the shaven dorsal skin of 3 male and 3 female rabbits. The product was administered undiluted and the time of exposure in the patch test was 4 hours. According to the study, the test item 1,5,9 -cyclododecatrien showed a slightly irritant effect on the skin of male and female rabbits

.