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EC number: 401-540-3 | CAS number: 84632-65-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Nanomaterial Zeta potential
Administrative data
- Endpoint:
- nanomaterial Zeta potential
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Principle of test: (electrophoretic) light scattering with Malvern Zetasizer Nano ZS (Malvern Instruments GmbH, Herrenberg, Germany)
- GLP compliance:
- no
- Type of method:
- electrophoresis
- Details on methods and data evaluation:
- The zeta potential of the test item at the pH-values relevant for the test (4, 7, 9) was determined using a Malvern Zetasizer Nano ZS (Malvern Instruments GmbH, Herrenberg, Germany). The reference material Zeta Potential Transfer Standard (- 42 mV ± 4.2 mV, DTS1235, Malvern Instruments, Germany) was analyzed as quality assurance sample before and after the test item sample measurements (tolerance with 10 % instrument uncertainty: -47.12 - -37.04 mV).
An amount of 10.51 mg of PR 254 (nanoform) was weighed into a 250 mL glass beaker and mixed with 1 mL of UHQ for pre-wetting (overnight). After 24 h, to the pre-wetted paste 149 mL of UHQ were added achieving a nominal concentration of 70.07 mg/L. This mixture was then sonicated at 38 % amplitude for 10 min using an ultrasonic probe (HD4200, Bandelin).
For analysis the test item dispersion was diluted to the extent that it still shows a coloration. For zeta potential analysis the test item dispersion was diluted 2.6-fold in UHQ-water + 5 mM NaHCO3, adjusted to pH 4, 7 and 9 and analyzed with a nominal concentration of 26.3 mg/L and a particle number concentration of 3.2 x 1014 particles/L. Table 1 shows the measured zeta potentials of the test item at the different pH values - Sampling:
- - Short description of the method and its scientific and technical basis.
- Description of reference measurements and samples: The reference material Zeta Potential Transfer Standard (- 42 mV ± 4.2 mV, DTS1235, Malvern Instruments, Germany) was analyzed as quality assurance sample before and after the test item sample measurements (tolerance with 10 % instrument uncertainty: -47.12 - -37.04 mV).
- Technical equipment: Malvern Zetasizer Nano ZS (Malvern Instruments GmbH, Herrenberg, Germany)
- Reagents (if applicable): -
- Preparation of test samples and references (including the use of energy and dispersants): An amount of 10.51 mg of PR 254 (nanoform) was weighed into a 250 mL glass beaker and mixed with 1 mL of UHQ for pre-wetting (overnight). After 24 h, to the pre-wetted paste 149 mL of UHQ were added achieving a nominal concentration of 70.07 mg/L. This mixture was then sonicated at 38 % amplitude for 10 min using an ultrasonic probe (HD4200, Bandelin).
Test material
- Reference substance name:
- -
- EC Number:
- 401-540-3
- EC Name:
- -
- Cas Number:
- 84632-65-5
- Molecular formula:
- C18H10Cl2N2O2
- IUPAC Name:
- 3,6-bis(4-chlorophenyl)-1H,2H,4H,5H-pyrrolo[3,4-c]pyrrole-1,4-dione
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: D23508919P1 (red powder)
- Expiration date of the lot/batch: not available
- Purity test date: not available
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature, keep in original container; store container tightly closed in well-ventilated place
- Stability under storage conditions: stable
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/dispersant/vehicle/test medium: Only a small amount of the test substance entered the solution within the ultrasonic treatment.
- Reactivity of the test substance with the solvent/vehicle /test medium (if applicable): not applicable
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: pre-wetting overnightand sonification at 38 % amplitude for 10 min
- Preliminary purification step (if any): none
INFORMATION ON NANOMATERIALS
- see LE composition
Results and discussion
Zeta potentialopen allclose all
- Key result
- Zeta potential:
- -10.93 mV
- St. dev.:
- 0.29 mV
- pH:
- 4
- Key result
- Zeta potential:
- -26.6 mV
- St. dev.:
- 1.06 mV
- pH:
- 7
- Key result
- Zeta potential:
- -35.13 mV
- St. dev.:
- 0.45 mV
- pH:
- 9
Any other information on results incl. tables
Zeta Potential Transfer Standard (tolerance - 47.12 – - 37.04 mV):
- mean (n=3): -41.97
- SD (n=3): 1.36
Applicant's summary and conclusion
- Conclusions:
- The determined values for the zeta potential of PR 254 are negative in the investigated pH-range (pH 4 – 9). Furthermore, the data reveal that the zeta potential is lower at pH 4 than at pH 7 and 9. The data indicate that the zeta potential seems to decrease to a point of zero charge with possible charge reversal at lower pH-values. Thus, the dispersion of the nanoform seems to be stabilized to a certain extend towards agglomeration interactions at higher pH values, whereas at lower pH-values increased agglomeration can be expected.
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