Iron, complexes with diazotized 2-amino-4,6-dinitrophenol coupled with diazotized 4-nitrobenzenamine and 4-[(2,4-dihydroxyphenyl)azo]-5-hydroxy-2,7-naphthalenedisulfonic acid, sodium salts

Endpoint summary

Description of key information

OECD 404, similar substance 1, not skin irritant.

OECD 405, similar substance 1, eye irritant. H319

Key value for chemical safety assessment

Skin irritation / corrosion

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Endpoint conclusion

Endpoint conclusion:

adverse effect observed (irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin Irritation

Two studies were considered in order to complete the assessment on skin irritation: one test was conducted on the substance in itself nevertheless few information is available regarding the method and the experimental results. Therefore, another test conducted on the Similar Substance 01 was considered in order to confirm the results of the first test.

The two results are consistent (both not irritant) and the substance is expected to be not irritant.

Eye Irritation

Two studies were considered in order to complete the assessment on eye irritation: two tests were conducted on the substance in itself nevertheless few information is available regarding the methods and the experimental results.

Another study performed on similar substance 1 gave a eye irritation potential resutls, H319 Cat. 2. based on the read across justification same results apply to the target substance.

Justification for classification or non-classification

According to the CLP Regulation (EC n. 1272/2008) the following classifications apply:

 

Skin irritant cat2:

1) Mean value of ≥ 2,3 - ≤ 4,0 for erythema/ eschar or for oedema in at least 2 of 3 tested animals from gradings at 24, 48 and 72 hours after patch removal or, if reactions are delayed, from grades on 3 consecutive days after the onset of skin reactions; or

2)Inflammation that persists to the end of the observation period normally 14 days in at least 2 animals, particularly taking into account alopecia (limited area), hyperkeratosis, hyperplasia, and scaling; o

3) In some cases where there is pronounced variability of response among animals, with very definite positive effects related to chemical exposure in a single animal but less than the criteria above.

 Based on the results reported for skin irritation the substance is not classified.

 

Eye irritant Cat 2:

at least in 2 of 3 tested animals, a positive response of:

1)     corneal opacity ≥ 1 and/or

2)     2) iritis ≥ 1, and/or

3)      conjunctival redness ≥ 2 and/or

4)      conjunctival oedema (chemosis) ≥ 2

calculated as the mean scores following grading at 24, 48 and 72 hours after installation of the test material, and which fully reverses within an observation period of 21 days.

Based on the results reported for eye irritation the substance is classified as eye irritation H319 Cat. 2