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This study was performed to assess the acute toxicity of the test material to Daphnia magna. Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to a Water Accommodated Fraction (WAF) of the test material,
at a single nominal loading rate of 100 mg/l for 48 hours at a temperature of 22C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 24 and 48 hours. The 48-Hour EL*50 for the test material to Daphnia magna based on nominal loading rates was greater than 100 mg/l loading rate WAF and correspondingly the No Observed Effect Loading rate was 100 mg/l loading rate WAF.
A key GLP study reporting 48h EL 50 > 100 mg/l loading rate WAF, OECD, 202.
In a key GLP compliant study conducted in accordance with the OECD 202 guideline reported that the 48-Hour EL50 for the Daphnia Magna exposed to the test substance (WAF preparations) at the limit of water solubility, was greater than 100 mg/l in terms of the initial test substance loading rate. Correspondingly the No Observed effect Loading rate was 100 mg/l loading rate WAF.
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