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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

biodegradation in water: ready biodegradability
Adequacy of study:
other information

Data source

Reference Type:
other: Body responsible for the test

Materials and methods

Test guideline
according to guideline
Principles of method if other than guideline:
- Organization for Economic Co-operation and Development
(OECD), OECD guidelines for Testing of Chemicals, Section 3,
Degradation and Accumulation, guideline No. 301 B: "Ready
Biodegradability: CO2 Evolution Test" adopted July 17, 1992.

- European Community, Commission directive 92/69/EEC, Part
C: Methods for the determination of ecotoxicity, EC
Publication No. L383, December 1992, C.4. "Biodegradation:
determination of the 'ready' biodegradability, C.4-C: Carbon
dioxide (CO2) evolution test (Modified Sturm Test).

- ISO Standard 9439 (Water Quality - Evaluation of ultimate
aerobic biodegradability of organic compounds in aqueous
medium - carbon dioxide evolution test (1999).
GLP compliance:

Study design

Reference substance
Reference substance:
other: Sodium acetate

Results and discussion

% Degradation
Sampling time:
28 d
Details on results:
Points of degradation plot (test substance):
1 % degradation after 2 d
1 % degradation after 5 d
1 % degradation after 7 d
2 % degradation after 9 d
3 % degradation after 14 d
3 % degradation after 19 d
4 % degradation after 23 d
4 % degradation after 27 d
5 % degradation after 29 d

BOD5 / COD results

Results with reference substance:
Points of degradation plot (reference substance):
12 % degradation after 2 d
40 % degradation after 5 d
52 % degradation after 7 d
60 % degradation after 9 d
70 % degradation after 14 d
70 % degradation after 19 d
70 % degradation after 23 d
71 % degradation after 27 d
75 % degradation after 29 d

Applicant's summary and conclusion

Interpretation of results:
other: not readily biodegradable