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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study has been performed according to OECD and EC guidelines and according to GLP principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Principles of method if other than guideline:
OECD guidelines for Testing of Chemicals, guideline No. 202:
"Daphnia sp., Acute Immobilisation Test", Adopted April 13,
2004.


European Economic Community (EEC), EEC directive 92/69, Part
C: Methods for the determination of ecotoxicity,
Publication No. L383, December 1992, C.2. "Acute Toxicity
for Daphnia".


ISO International Standard 6341: "Water quality -
Determination of the inhibition of the mobility of Daphnia
magna Straus - Acute toxicity test, Third edition, 1996-04-
01.


Guidance document on aquatic toxicity testing of difficult
substances and mixtures, OECD series on testing and
assessment number 23, December 14, 2000.
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): TC4ASO2
- Substance type: multi-constituent
- Physical state: white powder
- Analytical purity: 97.8%
- Composition of test material, percentage of components: 92.9% 4-tert-Butylsulfonylcalix(4)arene (CAS 204190-49-8)
4.9% 4-tert-Butylsulfonylcalix(8)arene
- Lot/batch No.: AYUI-1Y
- Expiration date of the lot/batch: 07 October 2007
- Stability under storage conditions: Stable
- Storage condition of test material: At room temperature in the dark

Test solutions

Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: Test concentrations were prepared by stirring a dispersion
of the test substance in test medium (100 mg/l) for 72 hours
and subsequently filtering this mixture through a 0.45 mm
filter to obtain the water soluble fraction (WSF).
Additional test solutions (between 1% and 56%) were prepared
by dilution of various volumes of this filtrate in test
medium. All final test solutions were all clear and
colourless.

Test organisms

Test organisms (species):
other aquatic crustacea: DM

Study design

Test type:
static

Test conditions

Hardness:
180 mg CaCO3/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 0.08 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.08 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
%Concentration loss over test: ... 56

Any other information on results incl. tables

Comments:
Since 95% of the daphnids exposed to 100% WSF were recorded
as immobile in a combined limit/range-finding test, a final
test was performed exposing D. magna to 10, 18, 32, 56 and
100% of the WSF prepared at 100 mg/l. In contrast to the
range-finding test, exposure of D. magna to the 100% WSF did
not affect the mobility of more than 5% of the daphnids
exposed. In all other test solutions containing the WSF no
immobility of daphnids was recorded.


The initial concentration measured in the WSF was 0.18 mg/l
(final test), which decreased to 0.08 mg/l at the end of the
48-hour test period. Hence, the final concentration of 0.08
mg/l can be considered as the actual exposure concentration
in the final test.


The test substance did not induce acute immobilisation of
Daphnia magna, when these were exposed to a WSF prepared at
100 mg/l and corresponding with an actual concentration of
0.08 mg/l (NOEC).

Applicant's summary and conclusion