(Benzyloxycarbonyl)methyl 4-(1-Butyl-5-cyano-1,6-dihydro-2-hydroxy-4-methyl-6-oxo-3-pyridylazo)-benzoate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From July 15th, 2002 to August 15th, 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

1992

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Version / remarks:

1992

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Yellow 6314-PPT

IUPAC Name:

Yellow 6314-PPT

Test material form:

solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

other: % biodegradation = 100 × BOD / ThODNH4

Remarks:

Without nitrification

Value:

3

Sampling time:

28 d

% Degradation 2

Parameter:

other: % biodegradation = 100 × BOD / ThODNO3

Remarks:

With nitrification

Value:

2

Sampling time:

28 d

Details on results:

Points of degradation plot (test substance) based on ThOD_NH4:
1.5 % degradation after 7 d
2.5 % degradation after 14 d
3 % degradation after 21 d
3 % degradation after 27 d
3 % degradation after 28 d

Points of degradation plot (test substance) based on ThOD_NO3:
1 % degradation after 7 d
2 % degradation after 14 d
2 % degradation after 21 d
2 % degradation after 27 d
2 % degradation after 28 d

BOD5 / COD results

BOD5 / COD

BOD5 / COD 1

Parameter:

ThOD

Value:

1.75 g O2/g test mat.

Remarks on result:

other: without nitrification

BOD5 / COD 2

Parameter:

ThOD

Value:

2.26 g O2/g test mat.

Remarks on result:

other: with nitrification

Results with reference substance:

Points of degradation plot (reference substance) based on ThOD:
79 % degradation after 7 d
85.5 % degradation after 14 d
88.5 % degradation after 21 d
90 % degradation after 27 d
90 % degradation after 28 d

Any other information on results incl. tables

Biodegradadtion of reference item was 85.5 % at day 14, thus confirming the suitability of activated sludge. At the end of the test on day 28, degradation was 90 %.

The test item has no inhibitory effect on activated sludge microorganisms, as biodegradation rate in the toxicity control was > 25 % within 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test item was found to be not biodegradable under the test consitions within 28 days.