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Administrative data

Endpoint:
adsorption / desorption: screening
Remarks:
estimation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From July 22nd, 2002 to July 23rd, 2002
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Qualifier:
according to guideline
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Version / remarks:
August 2001
GLP compliance:
yes (incl. QA statement)
Type of method:
HPLC estimation method
Media:
soil/sewage sludge

Test material

Constituent 1
Reference substance name:
Yellow 6314-PPT
IUPAC Name:
Yellow 6314-PPT
Test material form:
solid
Radiolabelling:
no

Study design

Test temperature:
20 °C

HPLC method

Details on study design: HPLC method:
Column: LiChrospher 100 CN, part. size 5 μm, 250 mm × 4.6 mm
Mobile phase: 0.1 % phosphoric acid in water and acetonitrile (45:55 v/v); pH 3.7
Detection: 210 nm for reference items and for sodium nitrate, 448 nm for the test item solution
Temperature: 20 °C
Injection volume: 20 μl
Flow: 1.0 ml/min

Batch equilibrium or other method

Computational methods:
The adsorption coefficient (Koc) of the test item was interpolated from the calibraticn curve which was established by correlation of the log Koc values of various reference items with their log k' values. The capacity factor (k') is calculated from the retention time of the reference items, the test item and an unretarded item. Afterwards, a correlation of log k' versus log Kco of the reference items was plotted using linear regression. The log k' value of the test item was then used to calculate its log Koc value.

Results and discussion

Adsorption coefficientopen allclose all
Type:
Koc
Value:
451 561 dimensionless
Type:
log Koc
Value:
5.7 dimensionless

Results: HPLC method

Details on results (HPLC method):
Based on the chromatographic data, Yellow 6314-PPT was considered to be stable during the test procedure.

Applicant's summary and conclusion

Conclusions:
logKoc value is ca. 5.7; this value indicates that the test item is immobile and remains preferably in soil.

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