Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran

EC Number:

262-475-2

EC Name:

Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran

Cas Number:

60857-05-8

Molecular formula:

C10H16O

IUPAC Name:

4-methylidene-2-(2-methylprop-1-en-1-yl)oxane

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

female

Details on test animals or test system and environmental conditions:

Groups of 3 animals were held in stainless steel cages (31 cm x 46 cm x 19 cm) in rooms with air-condition (temperature 20 +/- 2°C, relative humidity 50 +/- 20%). The day/night rhythm was 12 h dark and 12 h night.
A standardized animal laboratory diet as well as drinking water were offered.

TEST ANIMALS
- Source: JANVIER supplier (France)
- Age at study initiation: about 7 weeks
- Acclimation period: at least 5 days
- Fasting period before study: yes

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

The animals, fasted prior to the test substance administration, were weighed again on D1 before administration.
The volume per kg of body weight, defined according to the test substance density (= 0.88) as equal to 2.27 ml/kg, the volumes of test substance were calculated for each rat.
After administration, animals were fasted for 3 to 4 hours.

Doses:

2000 mg/kg

No. of animals per sex per dose:

6

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: at least once a day for clinical signs; before test substance administration and 3, 7 and 14 days after administration for body weights
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

none

Clinical signs:

other: Slight toxicity signs were observed during the first 4 hours following treatment (piloerection and reduced motor activity in all the animals sometimes associated to hypotonia or porphyrin deposits around the muzzle); these signs have totally disappeared

Gross pathology:

No organ or tissue lesion macroscopically visible was found during the post mortem examination performed in all the animals 14 days after treatment.